Bc0385

The enhanced BCA protein assay kit, ATP and NADH content determination kit were purchased from Beyotime Institute of Biotechnology (catalog: P0012, S0026 and S0175, Shanghai, China). Fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), lipase (LPS), mitochondrial isocitrate dehydrogenase (ICDHm), NAD-malate dehydrogenase (NAD-MDH), succinate dehydrogenase (SDH), pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (α-KGDH), glutamine synthetase (GS), glutamate synthase (GOGAT), acetyl Co-A, and GSH assay kits were purchased from Beijing Solarbio Science & Technology Company Limited (Beijing, China), which catalog were BC0555, BC0415, BC2345, BC2165, BC1045, BC0955, BC0385, BC0715, BC0350, BC0075, BC0985, and BC1175, respectively. Chemical reagents were obtained by Shanghai Aladdin Bio-Chem Technology Company Limited (Shanghai, China). Deionized water (18.9 MΩ) was obtained by filtration of distilled water using a Milli-Q system (Millipore, MA, USA).

The enzyme activity of α-KGDH, CS, PC, and PDH was detected according to the instructions of the assay kit (BC0715, BC1060, BC0730, BC0385, Solarbio, Beijing, China). The enzyme activity was normalized to the protein content.