0.45 μm pore size filter devices

Manufactured by Merck Group

The 0.45-μm pore size filter devices are laboratory equipment designed to filter solutions or suspensions. The devices feature a pore size of 0.45 micrometers, which is suitable for the removal of particulates, microorganisms, and other small contaminants from the filtered material.

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Lab products found in correlation

Hitrap mabselect sure column, by GE Healthcare (2 mentions)

2 protocols using 0.45 μm pore size filter devices

Recombinant Monoclonal Antibody Production

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For recombinant mAb production, cDNA encoding the genes of heavy and light chains were cloned into DNA plasmid expression vectors encoding IgG1 or IgG1-LALA - or Fab- heavy chain (McLean et al., 2000 (link)) and transformed into E. coli cells. mAb proteins were produced after transient transfection of ExpiCHO cells following the manufacturer’s protocol and were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ДKTA instrument using HiTrap MabSelect Sure column (GE Healthcare Life Sciences). Purified mAbs were buffer exchanged into PBS, filtered using sterile 0.45-μm pore size filter devices (Millipore), concentrated, and stored in aliquots at −80°C until use.

Gilchuk P., Murin C.D., Milligan J.C., Cross R.W., Mire C.E., Ilinykh P.A., Huang K., Kuzmina N., Altman P.X., Hui S., Gunn B.M., Bryan A.L., Davidson E., Doranz B.J., Turner H.L., Alkutkar T., Flinko R., Orlandi C., Carnahan R., Nargi R., Bombardi R.G., Vodzak M.E., Li S., Okoli A., Ibeawuchi M., Ohiaeri B., Lewis G.K., Alter G., Bukreyev A., Saphire E.O., Geisbert T.W., Ward A.B, & Crowe JE J.r. (2020). Analysis of a Therapeutic Antibody Cocktail Reveals Determinants for Cooperative and Broad Ebolavirus Neutralization. Immunity, 52(2), 388-403.e12.

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Recombinant Monoclonal Antibody Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

For recombinant mAb production, cDNA encoding the genes of heavy and light chains were cloned into DNA plasmid expression vectors encoding IgG1 or IgG1-LALA - or Fab- heavy chain (McLean et al., 2000 (link)) and transformed into E. coli cells. MAb proteins were produced after transient transfection of ExpiCHO cells following the manufacturer’s protocol and were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA instrument using HiTrap MabSelect Sure column (GE Healthcare Life Sciences). Purified mAbs were buffer exchanged into PBS, filtered using sterile 0.45-μm pore size filter devices (Millipore), concentrated, and stored in aliquots at −80°C until use.

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