Cell proliferation was examined using CCK-8 kit (No. DD546592, Yiji, Shanghai, China) following the instructions. Briefly, each group of ovarian cancer cells were plated at a density of 1 × 104 cells per well in 96-well plates with 10% FBS, and then cultured in incubators with 37 °C, 5% CO2 for 48 h. A total of 10 μl of CCK-8 was added to each well at 0 h, 24 h, 48 h, 72 h and 96 h, and 96-well plates were incubated for additional 1 h, then the absorbance (OD) at 450 nm wavelength was measured by enzyme labeling instrument (No. DXT-11008412001, Roche, USA).
Dxt 11008412001
Manufactured by Roche
Sourced in United States
The DXT-11008412001 is a versatile lab equipment designed for a variety of applications. It offers reliable performance and precise measurements to support research and testing activities. The core function of this product is to provide accurate and consistent data collection to support scientific investigations.
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Lab products found in correlation
2 protocols using dxt 11008412001
1
Quantification of Cell Proliferation
2
Ovarian Cancer Cell Proliferation Assay
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Each group of cells were collected and lysed in RIPA buffer (No. 2114-100, BioVision, USA) supplemented with protease inhibitor cocktail (No. S25910, Yeyuan, Shanghai, China). The concentration was determined using a BCA protein assay kit (No. orb-EHJ033662, BIOHJSW, USA). Equal amount of the extracts were loaded and subjected to SDS-PAGE (No. YB100915-12, Ybscience, Shanghai, China), transferred onto nitrocellulose membranes (No. B500, ABM GOOD, CAN), and then blotted. Antibodies Cell proliferation was examined using CCK-8 kit (No. DD546592, Yiji, Shanghai, China) following the instructions. Brie y, each group of ovarian cancer cells were plated at a density of 1×10 4 cells per well in 96-well plates with 10% FBS, and then cultured in incubators with 37℃, 5% CO 2 for 48 h. A total of 10 μl of CCK-8 was added to each well at 0 h, 24 h, 48 h, 72 h and 96 h, and 96-well plates were incubated for additional 1 h, then the absorbance (OD) at 450 nm wavelength was measured by enzyme labeling instrument (No. DXT-11008412001, Roche, USA).
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