Burkholderia strains (Belgian Co-ordinated Collections of Micro-organisms) used in this study are listed in Table S1. Burkholderia and E. coli strains were routinely grown in LB broth (2.5%, MP Biomedicals) at 37 °C, with shaking at 200 rpm. Media were solidified with agar (1.5%, Invitrogen) and supplemented with filter-sterilized isopropyl-β-D-thiogalactopyranoside (IPTG, 25 μg/mL, ForMedium), ampicillin (100 μg/mL, Sigma-Aldrich), kanamycin (50 μg/mL, Sigma-Aldrich), or tetracycline (150 μg/mL, Sigma-Aldrich) when required. Bacterial strains were kept on plate at 4 °C, or stored at −80 °C in glycerol (25% v/v, Sigma-Aldrich).
Isopropyl β d thiogalactopyranoside
Manufactured by Formedium
Sourced in United Kingdom
Isopropyl-β-D-thiogalactopyranoside is a synthetic chemical compound commonly used as an inducer in molecular biology and biochemistry. It is a lactose analogue that can trigger the expression of genes under the control of the lac operon in bacteria.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using isopropyl β d thiogalactopyranoside
1
Cultivation and Maintenance of Burkholderia Strains
2
Bacterial Growth Media and Strain Maintenance
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Strains used in this study are listed in Table S1 . E. coli was grown in LB (MP Biomedicals) at 37°C, whereas Pseudomonas strains were grown with shaking at 200 rpm in Trypticase Soy Broth (TSB, BD Biosciences) and Casamino Acids Medium (CAA, BD Biosciences) at 30°C, except for P. aeruginosa that was grown at 37°C. Growth media were supplemented with agar (1.5%, Invitrogen), filter-sterilized isopropyl-β-D-thiogalactopyranoside (IPTG, 20 μg/ml, ForMedium), kanamycin (50 μg/ml, Sigma-Aldrich), or tetracycline (15–150 μg/ml, Sigma-Aldrich) when required. Strains were kept on plate at 4°C, or at −80°C in glycerol (25% v/v, Sigma-Aldrich).
3
Bacterial Growth Media Preparation
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Growth media, ampicillin, 5-aminolevulenic acid and isopropyl-β-D-thiogalactopyranoside (IPTG) were purchased from Formedium, Ltd. (Hunstanton, UK). Chemicals used in the preparation of phosphate buffers were purchased from Fisher Scientific (Loughborough, UK). Voriconazole was purchased from Discovery Fine Chemicals (Dorset, UK). Palmitoleic acid (C16:1) was purchased from Tokyo Chemical Industry UK Ltd (Oxford, UK). All other fatty acids and chemicals were purchased from Sigma-Aldrich (Poole, UK), unless otherwise stated.
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