Bicinchoninic acid (BCA) total protein kit, Earle’s Balanced Salt Solution (EBSS) and Dulbecco’s Modified Eagle’s Medium Nutrient Mixture F-12 Ham (DMEM, pH 7.2–7.4, without phenol red) with L-glutamine and 15 mM HEPES were obtained from GIBCO (Thermo Fisher Scientific, Rockford, IL, USA). DMEM was supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin. Phosphate buffered solution (PBS, pH 7.4) and lyophilized lipopolysaccharide (LPS) of Escherichia coli, serotype 0111:B4 were purchased from Lonza (Verviers, Belgium). WST-1 assay kit was obtained from Roche (Mannheim, Germany). Low-gelling agarose and protease inhibitor cocktail P1860 were obtained from Sigma Aldrich (Munich, Germany).
Phosphate buffered solution pbs
Manufactured by Lonza
Sourced in Belgium, Switzerland
Phosphate buffered solution (PBS) is a commonly used buffer solution in various laboratory applications. It is a balanced salt solution that maintains a stable pH, typically around 7.4, making it suitable for maintaining the physiological environment of cells and tissues. PBS is used as a diluent, washing solution, and for general purpose buffering in a wide range of experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Thermo Fisher Scientific (1 mentions)
Wst 1 assay kit, by Roche (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Gelatin type a, by Merck Group (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
Chitosan, by Merck Group (1 mentions)
Acs reagent, by Merck Group (1 mentions)
Glutaraldehyde solution grade 1 25 in h2o, by Merck Group (1 mentions)
2 protocols using phosphate buffered solution pbs
1
BCA-based Protein Quantification in Cell Cultures
2
Chitosan-Gelatin Nanocomposite Antibacterial Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Chitosan “medium molecular weight and 75–85% deacetylation”,
gelatin “type A obtained from porcine skin”, and glutaraldehyde
solution [grade I, 25% in H2O] were purchased from Sigma
Aldrich. Glacial acetic acid, 99.7% [ACS Reagent, M = 60.05 g/mol], and absolute ethanol [95%] were purchased from Sigma
Aldrich, Germany. Phosphate-buffered saline (PBS) pH 7 was purchased
from Oxford, India.
Both Difco LB broth medium and agar were
purchased from Beckman Dickinson Company. Dulbecco’s modified
Eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin EDTA,
penicillin/streptomycin, and phosphate-buffered solution (PBS) were
obtained from Lonza, Switzerland. S. aureus (ATCC 6538), “a Gram-positive bacteria”, and E. coli (ATCC 8739), “a Gram-negative bacteria”,
were used to test antibacterial properties of some selected CS/GL
nanocomposite NFs. Both strains were obtained from the Biotechnology
laboratories at The American University in Cairo.
Sterile 24-well
plates, tissue culture pipettes (5 and 10 mL),
T 25 cm2, T 75 cm2, and pipette tips all were
purchased from Grenier Bio-One, Austria.
gelatin “type A obtained from porcine skin”, and glutaraldehyde
solution [grade I, 25% in H2O] were purchased from Sigma
Aldrich. Glacial acetic acid, 99.7% [ACS Reagent, M = 60.05 g/mol], and absolute ethanol [95%] were purchased from Sigma
Aldrich, Germany. Phosphate-buffered saline (PBS) pH 7 was purchased
from Oxford, India.
Both Difco LB broth medium and agar were
purchased from Beckman Dickinson Company. Dulbecco’s modified
Eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin EDTA,
penicillin/streptomycin, and phosphate-buffered solution (PBS) were
obtained from Lonza, Switzerland. S. aureus (ATCC 6538), “a Gram-positive bacteria”, and E. coli (ATCC 8739), “a Gram-negative bacteria”,
were used to test antibacterial properties of some selected CS/GL
nanocomposite NFs. Both strains were obtained from the Biotechnology
laboratories at The American University in Cairo.
Sterile 24-well
plates, tissue culture pipettes (5 and 10 mL),
T 25 cm2, T 75 cm2, and pipette tips all were
purchased from Grenier Bio-One, Austria.
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