Cells were inoculated in 6-well plates before actinomycin D (AdooQ Bioscience, USA) treatment. All cells were treated with 2 μg/mL actinomycin D for 4 h, 8 h, 12 h and 24 h, whereas the control group was without any treatment. Then, RNA was extracted and subjected to qPCR detection of RNA stability.
Actinomycin d
Manufactured by Adooq
Sourced in United States
Actinomycin D is a laboratory-grade chemical compound used in scientific research. It is a natural product derived from the bacterium Streptomyces parvulus. Actinomycin D is known for its ability to inhibit DNA-dependent RNA synthesis, making it a useful tool in various biological and biochemical studies.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by Avantor (2 mentions)
Cisplatin, by Avantor (2 mentions)
Ethidium bromide, by Avantor (2 mentions)
Netropsin, by Avantor (2 mentions)
Hoechst 33342, by Avantor (2 mentions)
Streptavidin biosensors and kinetics buffer, by Molecular Devices (2 mentions)
Doxorubicin, by Thermo Fisher Scientific (2 mentions)
3 protocols using actinomycin d
1
Actinomycin D Regulation of RNA Stability
2
Characterization of DNA-binding compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Actinomycin D (1) was purchased from AdooQ Bioscience (Irvine, CA). Doxorubicin (2) was purchased from ThermoFisher Scientific (Waltham, MA). Ethidium bromide (3), propidium iodide (4), Hoechst 33342 (5), netropsin (6), and cisplatin (7) were purchased from VWR (Radnor, PA).
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
3
Characterization of DNA-binding compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Actinomycin D (1) was purchased from AdooQ Bioscience (Irvine, CA). Doxorubicin (2) was purchased from ThermoFisher Scientific (Waltham, MA). Ethidium bromide (3), propidium iodide (4), Hoechst 33342 (5), netropsin (6), and cisplatin (7) were purchased from VWR (Radnor, PA).
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
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