Milli q integral 10

Rice samples varying in amylose content were used. High-amylose rice cultivars #1 and #2 (HA1 and HA2, respectively) were grown at the Central Region Agriculture Research Center, NARO (Niigata, Japan). Momiroman (Momi) was obtained from a local farmer. Other rice cultivars and varieties, Yumetoiro (Yume), Koshihikari (Kosh), Milky Queen (Milk), Koganemochi (Koga), Basmati Rice (Basm), and Jasmine Rice (Jasm) were purchased at local markets. Isoamylase from Pseudomonas sp. was purchased from Megazyme International Ireland Ltd. (Wicklow, Ireland). All other reagent grade chemicals were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Purified water (produced with a Milli-Q Integral/10, Merck-Millipore Ltd., Burlington, MA, USA) was used in all experiments.
Structural properties of the rice were characterized as follows: apparent amylose content was determined by iodine colorimetry, ¹²⁾ amylose content by Concanavalin A (ConA) precipitation method using an amylose/amylopectin assay kit (Megazyme International Ireland), chain length distribution of amylopectin by high-performance anion exchange chromatography on a CarboPac PA-1 column equipped with a pulsed amperometric detector (HPAEC-PAD, Thermo Scientific Inc., Waltham, MA, USA) according to Nagamine and Komae (1996). ¹³⁾

Synthetic human Aβ_1-38, Aβ_1-40, Aβ_1-42, and Aβ_1-43 were purchased from the Peptide Institute (Osaka, Japan). Uniformly stable isotope-labeled peptides manufactured by rPeptide (Watkinsville, GA, USA), corresponding to each peptide (Aβ_1-38, Aβ_1-40, Aβ_1-42, and Aβ_1-43), were used as internal standards (ISs). Artificial CSF was purchased from Tocris Bioscience (Bristol, UK), and rat plasma was purchased from Charles River Laboratories Japan (Yokohama, Japan). Other materials and reagents were purchased from Kanto Chemical (Tokyo, Japan), Kokusan Chemical (Tokyo, Japan), and Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was prepared using Milli-Q Integral 10 (Merck, Darmstadt, Germany).