The largest database of trusted experimental protocols

Isotype control mab

Manufactured by Thermo Fisher Scientific

Isotype control monoclonal antibodies (mAbs) are used in flow cytometry and other immunoassays to establish appropriate staining controls. These mAbs are designed to bind to non-relevant antigens and serve as a baseline to distinguish specific antibody binding from non-specific background signal.

Automatically generated - may contain errors

2 protocols using isotype control mab

1

Flow Cytometry Immunophenotyping Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
FITC-conjugated mAbs to CD86 [clone 2331 (FUN-1)], CD274 (clone MIH1), PE-conjugated mAbs to pSTAT3 (clone 4/P-STAT3), CD80 (clone L307.4), PD-L2 (clone 2D3/B7-H2), antigen-presenting cell (APC)-conjugated mAbs to HLA-DR (clone G46-6), PD-1 (clone MIH4), Alexa 700-conjugated mAb to CD4 (clone RPA-T4), and BV421-conjugated mAb to CD4 were from BD Biosciences (Le Pont de Claix, France). PE-conjugated mAbs to IL-17A (clone eBio64CAP17), human–mouse RORγt (AFKJS-9), APC-conjugated mAb to FoxP3 (clone 236A/E7), and Fixable Vibility Dye eFluor® 506 were from eBioscience (Paris, France). PE-conjugated mAb to CD40 (clone MAB89) was from Beckman Coulter (Villepinte, France). Blocking mAb to human PD-L1 (clone MIH1) and isotype control mAb were from eBioscience. Alexa-488 conjugated mAb to IL-10 (clone JES59D7) and blocking mAb to PD-1 (clone EH12.2H7) were from Biolegend (London, UK).
+ Open protocol
+ Expand
2

Flow Cytometry Analysis of BMNC

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMNC isolated as described28 were stained with anti-CD3, anti-CD4, anti-CD8, anti-CD11b, anti-CD11c, anti-CD45, anti-F4/80, anti-Gr-1 or appropriate isotype control mAb (all from eBiosciences). CFSE (0.7 mg in 0.3 ml PBS-DMSO 7%; Santa Cruz Biotechnologies) or vehicle were injected i.v. on day 5 post-infection as reported3 (link). BMNC were then collected and stained with anti-CD11b, anti-CD11c or isotype control mAb (eBiosciences) and examined for expression of CFSE. Splenocytes were incubated with or without anti-CD3 mAb and Brefeldin A (10 μg/ml; eBiosciences). Cells were stained with anti-CD3, anti-CD4 and anti-CD8 (eBiosciences), permeabilized, stained with anti-IFN-γ or isotype control mAb (eBiosciences) and analyzed in an LSR II flow cytometer (BD Biosciences).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!