Ets 1 antibody

H9C2 cardiomyoblast cell line, cell culture medium, retinoic acid, Tween 20, sodium oleate and other reagents were purchased from Sigma Aldrich. Antibodies raised against phospho-AKT pSer473, Phospho-IRS-1 pSer307, NF-kB2, and IL-1 DMEM/Glutamax medium; BCA Protein Assay Kit; RIPA buffer; phosphatase inhibitor cocktail; Trizol reagent; High Capacity Reverse Transcriptions Kit, TaqMan Advanced miRNA cDNA Synthesis Kit, miRNAs Advanced Taqman assays, lipofectamine, and RNAimax were purchased from Thermo Fisher Scientific, Inc. Antibodies raised against IRS-1, AKT, and -Actin anti-rabbit IgG HRP-linked antibodies were purchased from Cell Signaling Technology, Inc.
Heat-inactivated fetal bovine serum was purchased from PAN Biotech. I-Taq Universal SybrGreen Master Mix, protease inhibitor cocktail (BioRad), rno-pre-miR-193b and control pre-miR were purchased from Exiquon. PVDF membranes were obtained from Amersham. Immobilon Chemiluminescent Western HRP Substrate was obtained from Millipore. High Pure miRNA Isolation Kit was purchased from Roche Diagnostics, and Magnetic Luminex Screening Assay was purchased from R&D Systems. Caspase-1 antibody was obtained from Santa Cruz Biotechnology. ETS-1 antibody was obtained from Bethyl laboratories. NLRP3 antibody was obtained from Novus Biologicals. TXNIP antibody was obtained from Abgent.