Aldosterone elisa kit

Electrolytes and other parameters of kidney function in blood were carried out by SA Pathology (Adelaide, Australia). Urine osmolality was measured using an Advanced 3320 osmometer (Advanced Instruments) and other parameters using an Advia 2400 chemistry system (Siemens). Serum aldosterone levels were measured using an Aldosterone Elisa Kit (Abcam, ab136933) and analyzed on a Spark 10 M microplate reader (Tecan, Switzerland).

Urine was collected from each mouse, held in an individual metabolic cage (Tecniplast, Exton, PA, USA), over a 24-h period. Blood samples were collected from the left ventricle before sacrificing under anesthesia. Serum samples were centrifuged for 5 minutes at 860 G and were stored at −80°C until analysis. Urine samples were also stored at −80°C for subsequent analysis.
Serum urea nitrogen and creatinine levels were measured using a Fuji Dri-Chem 3500 system (FUJIFILM Japan, Tokyo, Japan). Serum calcium and phosphorus levels were measured using Calcium E-Test Wako and Phospha C-Test Wako (FUJIFILM Wako Pure Chemical Industries, Osaka, Japan), respectively. Serum intact parathyroid hormone (iPTH), intact fibroblast growth factor 23 (iFGF23), and aldosterone levels were measured using ELISA (enzyme-linked immunosorbent assay) kits (mouse PTH 1-84 ELISA kit: Immutopics International, San Clemente, CA, USA; FGF23 ELISA kit: KAINOS laboratories, Inc., Tokyo, Japan; Aldosterone ELISA kit: Abcam, Cambridge, UK).

Blood samples were centrifuged for 5 min at 3000 rpm and were stored at − 80 °C until analysis. Serum creatinine, blood urea nitrogen, albumin, total cholesterol, calcium, phosphorus, and glucose levels were measured using a Fuji Dri-Chem 3500 (Fujifilm Japan, Tokyo, Japan). Urinary albumin, creatinine, and neutrophil gelatinase-associated lipocalin (NGAL) levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (albumin: Nephrat, Exocell, Philadelphia, PA, USA; creatinine: Creatinine Assay Kit, Cayman Chemical, Ann Arbor, MI, USA; NGAL: Lipocain-2 Rat ELISA Kit, Abcam, Cambridge, MA, USA). The urinary 8-hydroxydeoxyguanosine (8-OHdG), a sensitive indicator of oxidative DNA damage, was determined using a sandwich ELISA kit (Japan Institute for Control of Aging, Shizuoka, Japan). Serum aldosterone levels were measured using an Aldosterone ELISA kit (Abcam, Cambridge, UK).