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Supersignal west pico chemilumenscence

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SuperSignal West Pico Chemiluminescence is a sensitive detection reagent that emits light upon reaction with proteins labeled with horseradish peroxidase (HRP). It is used for the detection of proteins in Western blot analysis.

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Lab products found in correlation

β actin, by Merck Group (2 mentions) Supersignal west femto chemilumenscence substrate, by Thermo Fisher Scientific (2 mentions) Protein assay dye reagent, by Bio-Rad (1 mentions) Notch1 val1744 antibody, by Cell Signaling Technology (1 mentions) Ps473 akt, by Cell Signaling Technology (1 mentions) Pt202 y204 erk, by Cell Signaling Technology (1 mentions) Anti mouse hrp na931v, by GE Healthcare (1 mentions) Anti rabbit hrp na934v, by GE Healthcare (1 mentions) Sc 12984, by Santa Cruz Biotechnology (1 mentions) C ebpα, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

SuperSignal West Pico (704 citations)

2 protocols using supersignal west pico chemilumenscence

1

Notch1 Signaling Pathway Analysis

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Nuclear lysates were prepared using the Active Motif nuclear complex coimmunoprecipitation kit (54001), and whole-cell lysates were prepared with RIPA buffer; protease inhibitors were included for both. Protein concentration was determined with the Bio-Rad protein assay dye reagent (Bio-Rad). Proteins were separated using SDS-PAGE and transferred to PVDF membranes. Antibodies used for Western blot were cleaved Notch1 (Val1744) antibody (Cell Signaling Technology, no. 2421), MAML1 (D3E9) rabbit mAb (Cell Signaling Technology, no. 11959), β-actin (Sigma), and secondary anti-mouse-HRP (Pierce) or anti-rabbit-HRP (Pierce). Blots were visualized with SuperSignal west pico chemilumenscence (Thermo Scientific) or SuperSignal west femto chemilumenscence substrate (Thermo Scientific).
Gerhardt D.M., Pajcini K.V., D’altri T., Tu L., Jain R., Xu L., Chen M.J., Rentschler S., Shestova O., Wertheim G.B., Tobias J.W., Kluk M., Wood A.W., Aster J.C., Gimotty P.A., Epstein J.A., Speck N., Bigas A, & Pear W.S. (2014). The Notch1 transcriptional activation domain is required for development and reveals a novel role for Notch1 signaling in fetal hematopoietic stem cells. Genes & Development, 28(6), 576-593.
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2

Western Blot Analysis of Cellular Signaling

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Whole-cell lysates were prepared with RIPA buffer supplemented with protease inhibitors (Roche), NaF (10mM), PMSF (1mM) and Na3VO4 (1mM) or cells were directly lysed in SDS sample buffer. Proteins were separated using SDS-PAGE and transferred to PVDF membranes. Antibodies used for immunoblot from Cell Signaling Technology were pS473 AKT (4051), AKT (4691), pT202/Y204 ERK (9101), ERK (9102), Trib2 (13533), and C/EBPα (8178). The antibody against TAL1 was purchased from Santa Cruz Biotechnology (sc-12984). β-actin (A5316; Sigma) was used as a loading control. Secondary anti-mouse-HRP (NA931V) and anti-rabbit-HRP (NA934V) were obtained from GE Healthcare. Blots were visualized with SuperSignal west pico chemilumenscence (34080; Thermo Scientific) or SuperSignal west femto chemilumenscence substrate (34095; Thermo Scientific).
Stein S.J., Mack E.A., Rome K.S., Pajcini K.V., Ohtani T., Xu L., Li Y., Meijerink J.P., Faryabi R.B, & Pear W.S. (2016). Trib2 Suppresses Tumor Initiation in Notch-Driven T-ALL. PLoS ONE, 11(5), e0155408.
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