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2 protocols using potassium hydrogen carbonate khco3

1

Synthesis and Quantification of N-(2,3-dihydroxypropyl)valine

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Glycidol (98%, CAS No. 556-52-5) was purchased from Acros Organics (Geel, Belgium). L-Valine-(13C5) (96–98% purity), RS-glyceraldehyde and sodium cyanoborohydride, used for the synthesis of the internal standard N-(2,3-dihydroxypropyl)-(13C5)valine, were obtained from Cambridge Isotope Laboratories, Inc (Tewksbury, MA, USA) and Sigma Aldrich (St Louis, MO, USA) respectively. Cyanoacetic acid and ammonium hydroxide were purchased from Fluka (Buchs, Switzerland). The analytical standard used for the calibration curve, fluorescein thiohydantoin of N-(2,3-dihydroxypropyl)valine (diHOPrVal-FTH), was synthesized previously within the research group [15 (link)]. Fluorescein isothiocyanate (FITC) was purchased from Karl Industries (Aurora, OH, USA) and potassium hydrogen carbonate (KHCO3) from Merck (Darmstadt, Germany). All other chemicals (analytical grade) were obtained from Sigma Aldrich (St Louis, MO, USA).
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2

Synthesis and Characterization of Electrode Materials

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Cu mesh (Alfa Aesar, 0.11 mm), cobalt(ii) sulphate heptahydrate (Co(SO4)2, 7H2O; Merck, ≥98%), nickel(ii) sulphate heptahydrate (Ni(SO4)2, 7H2O; Merck, ≥98%), potassium hydrogen carbonate (KHCO3; Merck, ≥99.5%), ammonium per-sulphate solution (Lobachemie, ≥98%) ammonia solution (Merck, ≥25%), nickel(ii) chloride hexahydrate (NiCl2·6H2O; Merck, ≥99%), chromium(iii) chloride hexahydrate (CrCl3·6H2O; Merck, ≥96%), manganese(ii) chloride tetrahydrate (MnCl2·4H2O; Merck, ≥96%), sodium hydroxide (NaOH; Merck, ≥97%), sodium dodecyl sulphate (SDS; Merck, ≥98%), potassium hydroxide pellets (KOH; Merck, ≥85%), ethanol (C2H5OH; Merck ≥99.9%), Toray carbon fiber paper (CFP; Alfa Aesar), commercial Pt/C (40 wt%, Merck), ammonium molybdate ((NH4)6Mo7O24; 97%, Merck), sodium chloride (NaCl; 99%, Merck), sodium hydrogen citrate (Na2C6H6O7; 98%, Merck), potassium hexachloroiridate (K2IrCl6; 99%, Sigma-Aldrich), nickel nitrate hexahydrate (Ni(NO3)2·6H2O; 98%, Merck), iron nitrate nonahydrate (Fe(NO3)3·9H2O; 98%, Merck) and Nafion perfluorinated resin solution (5 wt%, Merck) were used without further purification.
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