Total RNA was extracted from cells using the TaKaRa FastPure RNA kit (Takara Bio, Ohtsu, Japan), according to the manufacturer’s instructions. First-strand synthesis and RT-PCR were performed using the One-step SYBR PrimeScript RT-PCR kit (Takara Bio), according to the manufacturer’s protocol. Amplification and detection were performed using the Applied Biosystems 7300 Real-time PCR System (Applied Biosystems, Foster City, CA, USA). PCR primers were purchased from Qiagen. The change in expression of each target mRNA was calculated relative to the level of 18 S rRNA44 (link).
Takara fastpure rna kit
Manufactured by Takara Bio
Sourced in Japan
The TaKaRa FastPure RNA kit is a product designed for the rapid and efficient isolation of high-quality total RNA from a variety of biological samples. The kit utilizes a simple and reliable method to extract RNA, making it a useful tool for various research applications.
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2 protocols using takara fastpure rna kit
1
Quantitative RNA Expression Analysis
2
RT-PCR Analysis of mRNA Expression
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Total RNA was extracted from A549 cells using the TaKaRa FastPure RNA kit (Takara Bio, Ohtsu, Japan), according to the manufacturer’s instructions. First-strand synthesis and RT-PCR were performed using the One-step SYBR PrimeScript RT-PCR kit (TAKARA, Ohtsu, Japan), according to the manufacturer’s protocol. Amplification and detection were performed using the Applied Biosystems 7300 Real-time PCR System (Applied Biosystems, Foster City, CA). PCR primers were purchased from Qiagen. The change in expression of each target mRNA was calculated relative to the level of 18S rRNA47 (link)48 (link).
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