The microarray data used in this study were acquired from GEO database (http://www.ncbi.nlm.nih.gov/gds/ ), which is a public repository containing the high-throughput gene expression data submitted by research institutions around the world. We chose the mRNA expression profiles of GSE115810 and GSE36389 from the GEO database. The array data of GSE115810 was consisted with 24 EC tissues and 3 normal adjacent mucosa tissues based on the platform of GPL96 [HG-U133A] Affymetrix Human Genome U133A Array. The array data of GSE36389 was consisted with 13 EC tissues and 7 normal adjacent mucosa tissues based on the platform of GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version).
Agilent 014850 whole human genome microarray 4x44k
Manufactured by Agilent Technologies
The Agilent-014850 Whole Human Genome Microarray 4x44K is a DNA microarray product designed for comprehensive whole-genome expression analysis. It features 44,000 probes that cover the majority of the human genome. The microarray allows for the simultaneous measurement of the expression levels of thousands of genes in a single experiment.
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Lab products found in correlation
2 protocols using agilent 014850 whole human genome microarray 4x44k
1
Gene Expression Profiling of Esophageal Cancer
2
Transcriptomic Profiling of Pancreatic Ductal Adenocarcinoma
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PDAC microarray datasets GSE28735 (Affymetrix Human Gene 1.0 ST Array), GSE71729 (Agilent-014850 Whole Human Genome Microarray 4x44K), TCGA-PAAD (UNC_IlluminaHiSeq_RNASeqV2, level 3) and their corresponding clinical data in this study were directly downloaded from Gene Expression Omnibus (GEO) database (http://www.ncbi.nlm.nih.gov/geo/ ) and the open access tiers of the Cancer Genome Atlas (TCGA) data portal (https://gdc-portal.nci.nih.gov/ ). GSE28735 included 45 PDAC samples and 45 pared adjacent non-tumor tissue samples. GSE71729 analysis used data from 145 primary PDAC tumors and 46 pancreas tissues. Among 145 PDAC cases, 130 cases with clinical information were left for further analysis and 124 cases with overall survival information were left for Kaplan-Meier analysis. TCGA-PAAD RNA-Seq included 166 cases with overall survival information. Expression data extraction was performed with R 3.2.5 software. In order to gain insight into the biological mechanism involved in PDAC progression through FAM83B, gene set enrichment analysis (GSEA) was performed in tumor samples of GSE71729 datasets using the GO genesets database (c2.all.v4.0.symbols.gmt) from the Molecular Signatures Database-MsigDB.
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