ISH was performed by a conventional method with a few key reaction conditions. Samples were treated with 5 μg/ml Proteinase K (Takara, Japan) at 37 °C for five minutes, incubated with a probe (500 nM) at 50 °C for 1 h, and then washed with SSC (Sangon Biotech, China) at 50 °C for 20 min. An anti-DIG-AP reagent (Roche, Switzerland) was used at 4 °C for one night. NBT-BCIP (Roche, Switzerland) was used for colorimetric detection.
Anti dig ap reagent
Manufactured by Roche
Sourced in Switzerland
The Anti-DIG-AP reagent is a laboratory product designed for detection and analysis purposes. It functions as a conjugate that binds to digoxigenin (DIG) labels, enabling the visualization and identification of target analytes in various experimental and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anti dig ap reagent
1
Conventional In Situ Hybridization Protocol
2
In Situ Hybridization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ISH was performed by a conventional method with a few key reaction condi tions. Samples were treated with 5μg/ml Proteinase K (Takara, Japan) at 37°C for ve minutes, incubated with a probe (500 nM) at 50°C for one hour, and then washed with SSC (Sangon Biotech, China) at 50°C for 20 minutes. An anti-DIG-AP reagent (Roche, Switzerland) was used at 4°C for one night. NBT-BCIP (Roche, Switzerland) was used for colorimetric detection.
3
In Situ Hybridization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ISH was performed by a conventional method with a few key reaction condi tions. Samples were treated with 5μg/ml Proteinase K (Takara, Japan) at 37°C for ve minutes, incubated with a probe (500 nM) at 50°C for one hour, and then washed with SSC (Sangon Biotech, China) at 50°C for 20 minutes. An anti-DIG-AP reagent (Roche, Switzerland) was used at 4°C for one night. NBT-BCIP (Roche, Switzerland) was used for colorimetric detection.
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