Antibodies against ho 1

CAY10591 and SRT1720 were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Primary antibodies against β-actin, CREB (sc-25785), and NF-κΒ p65 (sc-7151) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against phospho-AMPK^Thr172 (#2535), acetyl-p53^Lys379 (#2570), phospho-p53^Ser15, phospho-mTOR^Ser2448, and phospho-CREB^Ser133 (#9198) were purchased from Cell Signaling Technology. The primary antibodies against iNOS (610431) and p53 were purchased from BD Transduction Lab (Lexington, KY, USA). The primary antibody against COX-2 (aa 570-598) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Antibodies against α-tubulin (T5168), acetylated tubulin^Lys40 (T7451), HDAC6, EX527 (E7034), and TSA (T8552) were purchased from Sigma Aldrich (St. Louis, MO). Antibodies against HO-1 were purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). Antibodies against β-actin (ab6267) and SIRT1 (ab50517) were purchased from Abcam (Cambridge, MA, USA).

Antibodies against HO-1 (Enzo, East Farmingdale, NY, USA), glutamate–cysteine ligase modifier (GCLM) subunit and MPO (Abcam, Cambridge, UK), NQO1 and Nrf2 (Santa Cruz Biotech, Santa Cruz, CA, USA), Prx-1 (Ab Frontiers, Seoul, Korea), and β-actin were purchased from commercial sources. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin, and streptomycin were purchased from HyClone (Logan, UT, USA). Oligonucleotides were purchased from Bioneer (Daejeon, Korea). Other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless indicated otherwise.
Tau-Cl was synthesized freshly on the day of use by adding equimolar amounts of NaOCl to taurine. The formation of Tau-Cl and its concentration were monitored by measuring UV absorption at 200–400 nm [32 (link)].

Luteolin was obtained from Wako Pure Chemical (Osaka, Japan). Antibodies against NQO1, CLOCK and PER2 were from Abcam (Cambridge, UK). Antibodies against β-actin and BMAL1 were from Cell Signaling Technology (Danvers, MA, USA). Antibodies against HO-1 (Enzo Life Sciences Inc., Farmingdale, NY, USA), lamin B (Santa Cruz Biotechnology, Dallas, TX, USA) and Nrf2 (Medical & Biological Laboratories, Aichi, Japan) were also used in this study. All other reagents used were of the highest grade available from a commercial source.