Xpert pro 3050 60

As prepared NP were characterized by using UV-Vis spectrometry UV-3101PC scanning spectrophotometer (Shimadzu, Japan) with a 1 cm quartz cell. The morphology and particle size of nanoparticle was analyzed by high-resolution transmission electron microscopy (HR-TEM, Tecnai G20, FEI, USA) under an accelerating voltage of 200 kV. The samples were prepared by placing a drop on the surface of 400-mesh carbon-coated Cu grid and dried under sodium lamp for ten min before examining. X-ray diffraction (XRD) was performed to verify the NP crystalline structure and presence of facet planes (Panalytical Xpert-PRO 3050/60). Samples were prepared by concentrating TSNP solution through centrifugation at 15 000 rpm for 12 min. The supernatant was removed from the sample. The process was repeated three times. Next, concentrated solution was added dropwise on the glass slide and allowed to dry at room temperature.

The physicochemical properties of nanoparticles are important for their
bio-distribution, safety, and efficacy. Characterization is performed using a
variety of analytical techniques, including Scanning Electron Microscopy (SEM
– JEOL JSM-7600F) for surface morphology and size of the nanoparticles.The
X-ray diffraction (XRD – D8 ADVANCE) measurement was performed on X-ray
diffractometer (Panalytical Xpert-PRO 3050/60) operated at 30 kV and 100 mA and
spectrum was recorded by CuKα radiation with wavelength of 1.5406 Å in
the 2θ range of 20°-80° andiron content determination was done
using Atomic Absorption Spectroscopy (AAS – PEAnalyst800) [6 (link)-12 (link, link, link, no link found, link, no link found)].

The phytosynthesized AgNPs were characterized by Ultraviolet–visible spectral analysis. The absorbance spectra were recorded using Ultraviolet–visible spectroscopy (UV-1800 Shimadzu UV spectrophotometer) from 300 to 700 nm. Fourier transform infrared spectroscopy was performed on a Thermo Scientific™ Nicolet iS™50 FTIR Spectrometer to detect possible functional groups in biomolecules present from leaf extracts of B. asiatica. X-ray diffraction was performed on a X-ray diffractometer (Panalytical Xpert-PRO 3050/60) operated at 30 kV and 100 mA and the spectrum was recorded by CuKα radiation with wavelength of 1.5406 Å in the 2θ range of 20°–80°. The surface morphology and size of the AgNPs were examined using a scanning electron microscope (SEM) on a NOVA-450 instrument. Transmission electron microscope (TEM), energy dispersive spectroscopy (EDS), and selected area electron diffraction (SAED) measurements were performed on a Tecnai G2 20 S-TWIN instrument. The atomic force microscopy (AFM) used a nanoscope8 in its non-contact mode. Viable bacteria cells were counted using a digital colony counter (LAB India).