The production of mitochondrial superoxide in mMSCs was measured using MitoSOX (Thermo Fisher Scientific). To measure mitochondrial membrane potential, tetramethylrhodamine, methyl ester, and perchlorate (TMRE; Thermo Fisher Scientific) dye was used. The mMSCs in each group were trypsinized and centrifuged at 1,200 rpm for 3 min. After washing with PBS twice, the cells were treated with either 10 μM MitoSOX in PBS or 200 nM TMRE in PBS at 37°C for 15 min. MitoSOX-positive or TMRE-positive cells were analyzed using flow cytometry (Sysmex, Kobe, Japan). flow cytometry data were analyzed using FCS Express 5 Flow research software (DeNovo Software, Los Angeles, CA, USA).
Fcs express 5 flow research software
Manufactured by De Novo Software
Sourced in United States
FCS Express 5 Flow is a flow cytometry data analysis software. It provides tools for data visualization, gating, and statistical analysis of flow cytometry experiments.
Automatically generated - may contain errors
2 protocols using fcs express 5 flow research software
1
Quantifying Mitochondrial Superoxide and Membrane Potential in mMSCs
2
Mitochondrial ROS and Membrane Potential
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each TH1 cell group was trypsinized and treated with 5 μM MitoSOX (Thermo Fisher Scientific) and 100 nM tetramethylrhodamine, methyl ester, and perchlorate (TMRE; Thermo Fisher Scientific) for 30 min. After washing twice with PBS, the samples were measured by flow cytometry (Sysmex, Kobe, Japan). MitoSOX- and TMRE positive cells were identified using FCS Express 5 Flow research software (De Novo Software, Los Angeles, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!