1 3 7 trimethyluric acid

Manufactured by Merck Group

Sourced in United States

1,3,7-trimethyluric acid is a chemical compound used in scientific research and laboratory settings. It is a purine derivative with a specific molecular structure. This compound may serve as a reference standard or have analytical applications, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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3 protocols using 1 3 7 trimethyluric acid

Analytical Standards for Tea Metabolites

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Formic acid and methanol (LC-MS grade) were purchased from TIC (Tokyo, Japan) and Merck (Darmstadt, Germany), respectively. Deionized ultrapure water was obtained from Millipore (Bedford, MA, USA). Flavanols of epi gallo catechin gallate (EGCG), epi gallo catechin (EGC), epi catechin gallate (ECG), epi catechin (EC), gallo catechin gallate (GCG), gallo catechin (GC), catechin gallate (CG), and catechin (C), 1,3,7-trimethyluric acid, kaempferol-3-glucoside, quercetin-3-glucoside, quercetin-3-galactoside, myricetin 3-galactoside, naringenin, valine, tryptophan, arginine, proline, theogallin, chlorogenic acid and 5-methoxysalicylic acid were obtained from Sigma (St. Louis, MO, USA). Theacrine, theobromine and caffeine were purchased from Yuanye (Shanghai, China). Procyanidin B1, procyanidin B2 and procyanidin B3 were gained from ChemFaces (Wuhan, Hubei, China). Epiafzelechin and epiafzelechin 3-gallate were obtained from Mreda (Beijing, China) and Asiabio (Basel, Switzerland), respectively. epi gallo catechin-3-O-(3″-O-methyl)-gallate (EGCG3″Me) and epi catechin-3-O-(3″-O-methyl)-gallate (ECG3″Me) were purchased from Nagara Science Co., Ltd. (Gifu, Japan). Phenylethyl primeveroside and benzyl primeveroside were synthesized by staff at the National Glycoengineering Research Center, Shandong University, Jinan, China (Li et al., 2019 (link)).

Wu W., Lu M., Peng J., Lv H., Shi J., Zhang S., Liu Z., Duan J., Chen D., Dai W, & Lin Z. (2022). Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua). Food Chemistry: X, 13, 100270.

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LC-MS Analysis of Methylxanthine Metabolites

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LC-MS grade methanol, water, acetonitrile and formic acid used during sample preparation and LC-MS/MS analysis were purchased from VWR chemicals (Vienna, AT). Xenobiotic and metabolite standards (caffeine, theobromine, theophylline, para xanthine, 1-methyl xanthine, 3-methyl xanthine, 7-methyl xanthine, 1-methyluric acid, 3-methyluric acid, 1,7-dimethyluric acid, 3,7-dimethyluric acid and 1,3,7-trimethyluric acid, chlorogenic acid, xanthine, 5-Acetylamino-6-formylamino-3-methyluracil, dopamine and proteinogenic amino acids) were either purchased from Sigma–Aldrich (Vienna, AT) or Honeywell Fluka (GER). caffeine capsules were bought from Mach dich wach! GmbH (GER). Sampling units were made from filter papers (precision wipes, number = 7552, white, 11 × 21 cm, Kimtech Science, Kimberly-Clark Professional, USA) using a circular puncher of 1cm².

Brunmair J., Gotsmy M., Niederstaetter L., Neuditschko B., Bileck A., Slany A., Feuerstein M.L., Langbauer C., Janker L., Zanghellini J., Meier-Menches S.M, & Gerner C. (2021). Finger sweat analysis enables short interval metabolic biomonitoring in humans. Nature Communications, 12, 5993.

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Targeted LC-MS/MS Metabolomics Workflow

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LC-MS grade methanol, water, acetonitrile and formic acid used during sample preparation and LC-MS/MS analysis were purchased from VWR chemicals (Vienna, AT). Xenobiotic and metabolite standards (caffeine, theobromine, theophylline, para xanthine, 1-methyl xanthine, 3methyl xanthine, 7-methyl xanthine, 1-methyluric acid, 3-methyluric acid, 1,7-dimethyluric acid, 3,7-dimethyluric acid and 1,3,7-trimethyluric acid, chlorogenic acid, xanthine, 5-Acetylamino-6-formylamino-3-methyluracil, dopamine and proteinogenic amino acids) were either purchased from Sigma Aldrich (Vienna, AT) or Honeywell Fluka (GER). caffeine capsules were bought from Mach dich wach! GmbH (Germany). Sampling units were made from filter papers (Kimtech Science) using a circular puncher of 1cm 2 .

Brunmair J., Niederstaetter L., Neuditschko B., Bileck A., Slany A., Janker L., Feuerstein M., Langbauer C., Gotsmy M., Zanghellini J., Meier S.M., & Gerner C. (2020). Finger Sweat Analysis Enables Short Interval Metabolic Biomonitoring in Humans.

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