Mda and gsh assay kit

MDA and GSH were measured using MDA and GSH Assay kit (Beijing Solarbio Science & Technology Co., Ltd.) according to the manufacturer's instructions. The production of MDA serves as an index of lipid peroxidation and gives a pink color once reacted with thiobarbituric acid with a maximum absorption at 535 nm. GSH assay is based on the GSH recycling system by GSH substrate (DTNB) and GSH reductase. DTNB and GSH react to generate 2-nitro-5-thiobenzoic acid which has a yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm. Optical density was measured at 532 nm for MDA or 412 nm for GSH by a microplate reader (Molecular Devices, LLC).

Malonyldialdehyde (MDA) and glutathione (GSH) contents were measured using MDA and GSH assay kit (Solarbio, Beijing, China) according to the manufacturer's instructions. Optical density was measured absorbance at 450 nm, 532 nm and 600 nm for MDA content or 412 nm for GSH content by a microplate reader (Molecular Devices, Sunnyvale, USA).