Antibodies against protein resistin (sc‐376336), TLR4 (sc‐293072), NF‐κB (sc‐372), PI3K p‐p85 (sc‐12929R), EGFR (sc‐377229), and phosphorylated (p‐)EGFR (sc‐12351) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Twist1 (WL0109), PI3K p85 (WL01169), Akt (WL0003b), c‐Src (WL01570), p‐Src (WL02114), and p‐Akt (WL03307) were purchased from Wanleibio (Shenyang, China). Antibodies against MMP2 (#4022S) and GST (#2622) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against β‐actin (A1978) was purchased from Sigma (St. Louis, MO, USA). Horseradish peroxidase‐conjugated goat anti‐rabbit IgG (AP132F) and goat anti‐mouse (401215) secondary antibodies were bought from Sigma. Recombinant human resistin and resistin with His‐tag were purchased from ProSpec (Rehovot, Israel). Agarose bead‐conjugated anti‐His mouse clonal antibody (AT0084) was purchased from CMCTAG (San Diego, CA, USA). SB203580 (p38 MAPK inhibitor) and LY294002 (PI3K inhibitor) were purchased from Beyotime (Jiangsu, China). Diphenyliodonium (reactive oxygen species [ROS] inhibitor) was purchased from Sigma. Erlotinib HCl (EGFR inhibitor) was purchased from Selleck Chemicals (Houston, TX, USA). TAK242 (TLR4 inhibitor) was purchased from MedChem Express (Monmouth Junction, NJ, USA).
Diphenyliodonium
Manufactured by Merck Group
Sourced in United States
Diphenyliodonium is a chemical compound that can be used as a reagent in organic synthesis. It is a solid, crystalline material that is stable at room temperature. Diphenyliodonium serves as a source of the phenyl group in certain types of chemical reactions.
Automatically generated - may contain errors
Lab products found in correlation
Turbofect, by Thermo Fisher Scientific (2 mentions)
Antibody against β actin a1978, by Merck Group (1 mentions)
Tak 242, by MedChemExpress (1 mentions)
Ly294002, by Beyotime (1 mentions)
Sb203580, by Beyotime (1 mentions)
Rosmarinic acid, by Merck Group (1 mentions)
Apocynin, by Merck Group (1 mentions)
Acetylcholine, by Merck Group (1 mentions)
Fibronectin, by Santa Cruz Biotechnology (1 mentions)
Ebselen, by Merck Group (1 mentions)
Recombinant human tgf β1 protein, by R&D Systems (1 mentions)
Primary antibodies against grp78, by Santa Cruz Biotechnology (1 mentions)
α sma, by Santa Cruz Biotechnology (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Alamar blue, by Thermo Fisher Scientific (1 mentions)
Calcein am, by Thermo Fisher Scientific (1 mentions)
Phloroglucinol, by Merck Group (1 mentions)
Collagenase type 1, by Worthington (1 mentions)
4 protocols using diphenyliodonium
1
Signaling Pathway Dissection: Resistin-Mediated Regulation
2
Vasodilation Signaling Pathway Evaluation
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Rosmarinic acid, acetylcholine (ACh), compound C,5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), apocynin, and diphenyliodonium were purchased from Sigma Chemical Co. (St. Louis, MO, USA); phenylephrine (PE) and l-N-nitro arginine methyl ester (L-NAME) were purchased from Aladdin Industrial Co. (Shanghai, China). The other reagents were of analytical purity.
3
Recombinant TGF-β1 Protein Assays
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Recombinant human TGF-β1 protein was obtained from R&D Systems (Minneapolis, MN, USA). 4-phenylbutyrate (4-PBA), n-acetyl-L-cysteine (NAC), ebselen, diphenyliodonium (DPI), thapsigargin 000000(TG), and 2′,7′-dichlorofluorescein diacetate (DCFH-DA) were procured from Sigma (St. Louis, MO, USA). Primary antibodies against GRP78, fibronectin, α-SMA, and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and XBP-1s antibody was obtained from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were obtained from Vector Laboratories (Burlingame, CA, USA).
4
Collagenase-Induced Hypoxia Signaling
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Collagenase type I was ordered from Worthington Biochemical Corporation. Isoform-specific rat monoclonal col4a1, col4a5 NC1 antibodies were purchased from Chondrex Inc. We used rabbit HIF1alpha antibody from Abcam (ab179483). The anti-actin antibody, phloroglucinol, diphenyliodonium were all purchased from Sigma. PFHR-9, a mouse embryonic carcinoma cell line was obtained from ATCC. For transfecting PFHR-9 cells, we used TurboFect from Thermo Scientific. AlamarBlue and calcein-AM were ordered from ThermoFisher Scientific.
Rabbit polyclonal anti-Nox4 antibody was a generous gift from Professor Ajay Shah (King's College, London) and polyclonal anti-Duox1 antibody was a kind gift from Dr. Xavier De Deken (Université Libre de Bruxelles).
Rabbit polyclonal anti-Nox4 antibody was a generous gift from Professor Ajay Shah (King's College, London) and polyclonal anti-Duox1 antibody was a kind gift from Dr. Xavier De Deken (Université Libre de Bruxelles).
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