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Cd3 alexa fluor 488

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The CD3-Alexa Fluor 488 is a fluorochrome-conjugated monoclonal antibody that binds to the CD3 antigen, which is a component of the T-cell receptor complex. This product can be used for the identification and enumeration of T cells in flow cytometry applications.

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Lab products found in correlation

Cd25 apc cy7, by BD (2 mentions) Alexa fluor 546 conjugated donkey anti mouse igg antibody, by Thermo Fisher Scientific (2 mentions) Cd44 percp cy5, by Thermo Fisher Scientific (2 mentions) Mouse anti myc antibody sc 40, by Santa Cruz Biotechnology (2 mentions) Cd8a pecy7, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm solution, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Facscanto flow cytometer, by BD (2 mentions) Perm wash buffer, by BD (2 mentions) Cd4 apc, by BD (2 mentions)

2 protocols using cd3 alexa fluor 488

1

Intracellular Myc Expression in Thymic T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
We collected cells from the thymi of 6-week old male and female wild type and N-Me knockout mice and performed surface membrane stainings using the following antibodies: CD3-Alexa Fluor 488 (BD Pharmingen-557666), CD8a-PE-Cy7 (eBioscience-53-6.7), CD44-PerCP-Cy5.5 (eBioscience-IM7), CD25-APC-Cy7 (BD Pharmingen-557658) and CD4-APC (BD Pharmingen-553051). After surface staining we fixed the cells and permeabilized them with BD Cytofix/Cytoperm solution (BD Biosciences), washed them with BD Perm/Wash buffer (BD Biosciences), and stained them with a mouse anti-Myc antibody (sc-40, Santa Cruz) and an Alexa Fluor 546-conjugated donkey anti-mouse IgG antibody (A11036, Invitrogen). We analyzed the intracellular levels of Myc in a FACSCanto flow cytometer (BD Biosciences) using FACSDiva software (BD Biosciences).
Herranz D., Ambesi-Impiombato A., Palomero T., Schnell S.A., Belver L., Wendorff A.A., Xu L., Castillo-Martin M., Llobet-Navás D., Cardo C.C., Clappier E., Soulier J, & Ferrando A.A. (2014). N-Me, a long range oncogenic enhancer in T-cell acute lymphoblastic leukemia. Nature medicine, 20(10), 1130-1137.
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2

Intracellular Myc Expression in Thymic T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
We collected cells from the thymi of 6-week old male and female wild type and N-Me knockout mice and performed surface membrane stainings using the following antibodies: CD3-Alexa Fluor 488 (BD Pharmingen-557666), CD8a-PE-Cy7 (eBioscience-53-6.7), CD44-PerCP-Cy5.5 (eBioscience-IM7), CD25-APC-Cy7 (BD Pharmingen-557658) and CD4-APC (BD Pharmingen-553051). After surface staining we fixed the cells and permeabilized them with BD Cytofix/Cytoperm solution (BD Biosciences), washed them with BD Perm/Wash buffer (BD Biosciences), and stained them with a mouse anti-Myc antibody (sc-40, Santa Cruz) and an Alexa Fluor 546-conjugated donkey anti-mouse IgG antibody (A11036, Invitrogen). We analyzed the intracellular levels of Myc in a FACSCanto flow cytometer (BD Biosciences) using FACSDiva software (BD Biosciences).
Herranz D., Ambesi-Impiombato A., Palomero T., Schnell S.A., Belver L., Wendorff A.A., Xu L., Castillo-Martin M., Llobet-Navás D., Cardo C.C., Clappier E., Soulier J, & Ferrando A.A. (2014). N-Me, a long range oncogenic enhancer in T-cell acute lymphoblastic leukemia. Nature medicine, 20(10), 1130-1137.
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