Rat pge2 elisa kit

TNF-α, PGE_2, and NFκB expressions were measured using the Rat TNF-α ELISA kit (catalog no: E-EL-R0019), Rat PGE₂ ELISA kit (catalog no: E-EL-0034), and Rat NFκB ELISA kit (catalog no: E-EL-R0674), respectively, in accordance with the manufacturer’s guidelines (Elabscience). Using the Silent Crusher M (Heidolph) apparatus, the tissues were homogenized at 15,000 RPM; after centrifugation, the supernatant was collected (for 1 h at 1,350 g). TNF-α, PGE2, and p-NFκB concentrations were measured using an ELISA microplate reader. (Ansari et al., 2019 (link)).

Dibutyltindichloride, maellicanhydride, 4-Nitro aniline, ethanol, dimethyl sulfoxide (DMSO), chloroform and normal saline acquired from Sigma-Aldrich, Germany. DTN compound was prepared in postgraduate chemistry research laboratory of Riphah Institute of Pharmaceutical Sciences (RIPS) Islamabad, Pakistan. Omeprazole, metronidazole purchased from Barrett Hodgson and Sanofi Aventis. Secondary antibodies procured from Abcam UK. Rat NF-ĸB ELISA kit (Catalog No. E-El-R0676), rat TNF-α ELISA kit (Catalog No. E-El-R0019), rat IL-6 (Catalog No. DY406) and rat IL-1β (Catalog No. ab100704) and rat PGE₂ ELISA kit (Catalog No. E-El-R0034) were purchased from Elabscience, China. All chemicals were used in experiments analytically approved (99% HPLC grade).

All standard chemicals used were purchased from verified sources. Brucine was purchased from Sigma CO, Merck. Normal saline, absolute ethanol, and chloroform were acquired from Sigma-Aldrich (St. Louis, MO, United States). Omeprazole and metronidazole were purchased from Barrett Hodgson and Sanofi Aventis, Pakistan. Abcam, United Kingdom, provided secondary antibodies. Elabscience, China, provided the rat NFB ELISA kit (catalog no. E-EL-R0674), rat TNF-α ELISA kit (catalog no. E-EL-ROO19), and rat PGE2 ELISA kit (catalog no. E-EL-R0034). All of the chemicals used in this experiment were of analytical quality.