Il 17r mice

Wild type C57Bl/6 and TNF^-/- mice on the C57Bl/6 background were purchased from Jackson Laboratories (Bar Harbor, ME). IL-17R^-/- mice on the C57Bl/6 background were a gift from Amgen, Seattle [14 (link)]. Mice were immunized with ID93 (0.5 μg) adjuvanted with GLA-SE or GLA-AF (5 μg) via intramuscular injection or intranasal delivery to both nostrils (25 μL total volume). Adjuvants were prepared as described previously [6 (link)]. Mice were anesthetized with ketamine and xylazine prior to intranasal immunization. Mice were immunized 3 times at 3 week intervals. All mice were maintained in specific pathogen-free conditions. After infection animals were maintained in BL3 containment. All procedures were approved by the IDRI institutional animal care and use committee.

Wild-type C57BL/6 (WT), TCRβxδ^−/− and IL-21R^−/− mice were obtained from Jackson Laboratory. IL-17R^−/− mice were kindly provided by Amgen. CBir1 Tg mice were maintained and bred in the Animal Facilities at University of Texas Medical Branch (UTMB). All experiments were reviewed and approved by the Institutional Animal Care and Use Committees of the UTMB. All mice are bred onto the C57BL/6 background. 8–12 weeks-old mice were used for all experiments. All the mice used in this study were gender-matched, and maintained under SPF conditions.