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Mouse il 1β elisa kit

Manufactured by Mlbio
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Sourced in China

The Mouse IL-1β ELISA kit is a quantitative in vitro assay designed for the measurement of mouse interleukin-1 beta (IL-1β) concentration in biological samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.

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4 protocols using mouse il 1β elisa kit

1

Comprehensive Cytokine and Antibody Profiling

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The supernatant and serum were collected and analyzed using a mouse IL‐1β ELISA kit (mlbio, ml301814), IL‐6 ELISA kit (mlbio, ml063160), IL‐10 ELISA kit (mlbio, ml037873), MCP‐1 ELISA kit (mlbio, ml037840), IgG ELISA kit (mlbio, ml037601), IgM ELISA kit (mlbio, ml063597), TNF‐α ELISA kit (mlbio, ml002095), C3 ELISA kit (mlbio, ml002033), and MBP ELISA kit (Jin Yibai, JEB‐12718). The absorbance of each well was measured at 450 nm.
Wu C., Pan Y., Wang L., Liu M., Tu P., Chen S., Shi L., Yan D., Ma Y, & Guo Y. (2023). Inhibition of HDAC6 promotes microvascular endothelial cells to phagocytize myelin debris and reduces inflammatory response to accelerate the repair of spinal cord injury. CNS Neuroscience & Therapeutics, 30(3), e14439.
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2

Cytokine and Alarmin Levels in Murine and Human Samples

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The levels of IL-1β, IL-6 and TNF-α in mouse serum were evaluated by Mouse IL-1β ELISA kit (mIC50300-1, mlbio, Shanghai, China), Mouse IL-6 ELISA kit (IC50325-1, mlbio, Shanghai, China), Mouse TNF-α ELISA kit (mIC50536-1, mlbio, Shanghai, China). Moreover, the levels of S100a8/a9 in mouse serum and human plasma were detected by Mouse S100a8/a9 ELISA kit (ml037985, mlbio, Shanghai, China) and Human S100a8/a9 ELISA kit (ml038517, mlbio, Shanghai, China).
Wang Y., Shi Y., Shao Y., Lu X., Zhang H, & Miao C. (2024). S100A8/A9hi neutrophils induce mitochondrial dysfunction and PANoptosis in endothelial cells via mitochondrial complex I deficiency during sepsis. Cell death & disease, 15(6).
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3

Biomarker Panel for Kidney Injury

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Serum concentrations of creatinine, urea nitrogen, angiotensin II, aldosterone, BNP, ALT, AST, KIM-1, NGAL, RBP4 were detected by using Creatinine Kit (BC4910, Solarbio), Blood Urea Nitrogen Kit (BC1535, Solarbio), Angiotensin II ELISA kit (ml002228, Mlbio), Aldosterone ELISA kit (ml037530, Mlbio), Brain Natriuretic Peptide EIA Kit (ml037594, Mlbio), ALT Kit (BC1555, Solarbio), AST Kit (BC1565, Solarbio), Mouse KIM-1 ELISA Kit (BC4910, Solarbio), Mouse NGAL ELISA Kit (ml002075, Mlbio), Mouse RBP4 ELISA Kit (ARG81756, Arigo). Plasma concentrations of IL-1β, IL-6 and TNF-α were measured by Mouse IL-1β ELISA Kit (ml301814, Mlbio), Mouse IL-6 ELISA Kit (ab222503, Abcam), Mouse TNF-α ELISA Kit (ab208348, Abcam). Serum levels of retinol and retinoic acid were measured by liquid chromatography/mass spectrometry analysis following the previous methods 24 (link), 25 (link).
Liu B., Shalamu A., Pei Z., Liu L., Wei Z., Qu Y., Song S., Luo W., Dong Z., Weng X, & Ge J. (2023). A novel mouse model of heart failure with preserved ejection fraction after chronic kidney disease induced by retinol through JAK/STAT pathway. International Journal of Biological Sciences, 19(12), 3661-3677.
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4

Cytokine Profiling in Mouse and Human Samples

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Mouse IL-1α ELISA kit (Ml002273, Mlbio), Mouse IL-1β ELISA kit (Ml063132, Mlbio), Mouse IL-6 ELISA kit (Ml063159, Mlbio), Mouse IL-8 ELISA kit (Ml063162, Mlbio), Mouse TNF-α ELISA kit (Ml002095, Mlbio), Human IL-1β ELISA kit (Ml028611, Mlbio), Human TNF-α ELISA kit (Ml037301, Mlbio) were used for cytokines measured, respectively, according to the manufacturer's instructions.
Fan W., Ding C., Liu S., Gao X., Shen X., Boevre M.D., Gao Z., Li M., Zhang S., Miao Y., Guan W., Liu G., Yan L., DeSaeger S., & Song S. (2021). Estrogen Receptor β Activation Inhibits Colitis by Promoting NLRP6-Mediated Autophagy.
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