Soybeans (Glycine max) were obtained from Mexico City in a local market. Pepsin from porcine gastric mucosa (EC 3.4.23.1; ≥250 units/mg solid), pancreatin from porcine pancreas (EC 232-468-9; 8 × USP), the tripeptide IPI, called diprotin A, human recombinant DPP-IV enzyme, porcine pancreatic α-amylase (EC 3.2.1.1), and rat intestine α-glucosidase (EC 3.2.1.20) were purchased from Sigma-Aldrich (Madrid, Spain). The Quantitative Colorimetric Peptide Assay kit was from Pierce™ (Rockford, IL, USA), and the chromogenic substrate (H-Gly-Pro-p-nitroaniline) was purchased from Enzo Life Sciences Inc. (Farmingdale, NY, USA).
Human recombinant dpp 4 enzyme
Manufactured by Merck Group
Sourced in Spain
Human recombinant DPP-IV enzyme is a purified protein derived from human cells. It functions as a protease enzyme that cleaves peptides at proline residues.
Automatically generated - may contain errors
Lab products found in correlation
Pepsin from porcine gastric mucosa, by Merck Group (2 mentions)
H gly pro p nitroaniline, by Enzo Life Sciences (2 mentions)
Porcine pancreatic α amylase, by Merck Group (2 mentions)
Pancreatin from porcine pancreas, by Merck Group (1 mentions)
Rat intestine α glucosidase, by Merck Group (1 mentions)
Soluble potato starch, by Merck Group (1 mentions)
Porcine bile extract, by Merck Group (1 mentions)
Pefabloc sc, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (1 mentions)
2 protocols using human recombinant dpp 4 enzyme
1
In Vitro Digestibility of Soybean
2
In Vitro Enzymatic Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Pepsin from porcine gastric mucosa (EC 3.4.23.1), pancreatin from porcine pancreas (EC 232-468-9), porcine bile extract, the serine proteases inhibitor Pefabloc ® SC, ACE (EC 3.4.15.1), fluorescein disodium (FL), 3-[4,5-dimethylthiazol-2-yl]-2,3-diphenyl tetrazolium bromide (MTT), dimethylsulfoxide (DMSO), human recombinant DPP-IV enzyme, potato soluble starch, and porcine pancreatic α-amylase (EC 3.2.1.1) were purchased from Sigma-Aldrich (Madrid, Spain). Fluorogenic substrate o-aminobenzoylglycyl-p-nitro-l-phenylalanyl-l-proline (Abz-Glyp-Phe(NO2)-Pro-OH) was from Bachem Feinchemikalien (Bubendorf, Switzerland), and chromogenic substrate (H-Gly-Pro-p-nitroaniline) from Enzo Life Sciences Inc. (Farmingdale, NY, USA). 2,2'-azobis (2-methylpropionamide)-dihydrochloride (AAPH) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were from Aldrich (Milwaukee, WI, USA). The rest of chemicals used were of HPLC grade.
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