Undifferentiated and differentiated iPSCs (passage 15) were assessed for pluripotency and lineage-specific markers by immunofluorescence staining. Cells fixed in 4 % paraformaldehyde (15 min, room temperature) were washed with DPBS, incubated with a permeabilization-blocking buffer composed of 0.1 % Triton X-100 (Sigma-Aldrich) and 2.5 % donkey/goat serum (Sigma-Aldrich) in DPBS (60 min, room temperature), followed by primary antibodies (Table 2 ) in the same buffer (overnight, 4°C). Following DPBS washes, cells were incubated with secondary antibodies (Table 2 ) in DPBS supplemented with 2.5 % donkey/goat serum (60 min, room temperature), washed more with DPBS, and counterstained with NucBlue (Thermo Fischer Scientific) (15 min, room temperature) before visualization using a Revolve Fluorescent Microscope (Echo Laboratories).
Donkey goat serum
Manufactured by Thermo Fisher Scientific
Donkey/goat serum is a laboratory product used as a source of proteins, growth factors, and other biomolecules. It is commonly used in cell culture applications to supplement growth media and support the proliferation and maintenance of various cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using donkey goat serum
1
Pluripotency and Lineage Characterization of iPSCs
2
Pluripotency and Lineage Characterization of iPSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Undifferentiated and differentiated iPSCs (passage 15) were assessed for pluripotency and lineage-specific markers by immunofluorescence staining. Cells fixed in 4 % paraformaldehyde (15 min, room temperature) were washed with DPBS, incubated with a permeabilization-blocking buffer composed of 0.1 % Triton X-100 (Sigma-Aldrich) and 2.5 % donkey/goat serum (Sigma-Aldrich) in DPBS (60 min, room temperature), followed by primary antibodies (Table 2 ) in the same buffer (overnight, 4°C). Following DPBS washes, cells were incubated with secondary antibodies (Table 2 ) in DPBS supplemented with 2.5 % donkey/goat serum (60 min, room temperature), washed more with DPBS, and counterstained with NucBlue (Thermo Fischer Scientific) (15 min, room temperature) before visualization using a Revolve Fluorescent Microscope (Echo Laboratories).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!