Qubit quantification platform dsdna hs assay kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Qubit Quantification Platform dsDNA HS Assay Kit is a fluorescence-based method for accurately quantifying double-stranded DNA (dsDNA) samples. The kit provides reagents and materials for performing sensitive and specific quantification of dsDNA samples in the range of 0.2-100 ng.

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Lab products found in correlation

Thunderbird sybr qpcr mix, by Toyobo (1 mentions) Dneasy powersoil kit, by Qiagen (1 mentions)

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Qubit dsDNA Assay Kit (153 citations) Qubit dsDNA assay (43 citations) Qubit dsDNA HS (29 citations) Qubit HS kit (27 citations) DsDNA HS assay (25 citations) Qubit Quantification Platform (7 citations) HS dsDNA kit (4 citations) Qubit quantification assay (3 citations) DsDNA Qubit (3 citations) DsDNA Qubit Kit (2 citations)

2 protocols using qubit quantification platform dsdna hs assay kit

Soil Microbial DNA Extraction and Quantification

Cited 1 time

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DNA was extracted from 0.5 g soil using the Soil DNA Isolation Kit (Mo Bio, Carlsbad, CA) according to the manufacturer's protocol. Extracted DNA was quantified using the Qubit Quantification Platform dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA) and stored at −30°C until use.
For the real time PCR to quantify the total bacterial communitieswe used 16S rRNA gene-specific primers (968F and 1401R) essentially as described previously [33] (link). Amplification reaction was carried out using Thunderbird SYBR qPCR mix (Toyobo, Osaka, Japan) according to the manufacture's protocol. Amplification reactions were as follows: 2 min at 95°C, 40 cycles of 15 sec at 95°C, 30 sec at 50°C, 30 sec at 72°C.

Sugiyama A., Ueda Y., Zushi T., Takase H, & Yazaki K. (2014). Changes in the Bacterial Community of Soybean Rhizospheres during Growth in the Field. PLoS ONE, 9(6), e100709.

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Soil Microbiome Response to Santhopine and Nicotine

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Three different concentrations of santhopine or nicotine solution (low, 50 nmol g -1 soil; middle, 250 nmol g -1 soil; high, 1000 nmol g -1 soil) were applied every 3 days for five times, as described previously (13) . Sterile water was applied as a control. Tubes were incubated at room temperature in the dark. The concentrations of santhopine or nicotine solution were adjusted every time to keep the soil water content ratio at 30%. After incubation, total DNA was extracted using DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. Extracted DNA was quantified using Qubit Quantification Platform dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA) and stored at -80°C.

Shimasaki T., Masuda S., Garrido‐Oter R., Kawasaki T., Aoki Y., Shibata A., Suda W., Shirasu K., Yazaki K., Nakano R.T., & Sugiyama A. (2020). Species-specific assembly of root-associated bacterial microbiota mediated by a combination of plant specialized metabolites.

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