Cwr 22rv1 22rv1

Manufactured by American Type Culture Collection

The CWR-22RV1 (22RV1) is a cell line derived from a human prostate carcinoma. It is a widely used model for prostate cancer research.

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Lab products found in correlation

Lncap, by American Type Culture Collection (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Lncap lncap fgc, by American Type Culture Collection (1 mentions) Hek293t 293t, by American Type Culture Collection (1 mentions) Mycoplasma pcr detection kit, by GeneCopoeia (1 mentions) Enzalutamide enz, by Selleck Chemicals (1 mentions) Du145, by American Type Culture Collection (1 mentions)

2 protocols using cwr 22rv1 22rv1

Cell Line Authentication and Culturing

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CWR-22RV1 (22RV1) and LNCaP cell lines were purchased from ATCC, authenticated every six months using short tandem repeat (STR) profiling, and frequently tested for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza). LNCaP95 cells were derived from LNCaP cells and cultured with 10% phenol red-free charcoal-stripped serum (CSS). 22RV1, LNCaP, and LNCaP-derived stable cell lines were generally cultured in RPMI-1640 medium with 10% FBS, which was replaced by the hormone-depleted medium (5% CSS) for 2–3 days before the subsequent assays.

Ahn W.S., Park S.J, & Lee S.Y. (2000). Production of Poly(3-Hydroxybutyrate) by Fed-Batch Culture of Recombinant Escherichia coli with a Highly Concentrated Whey Solution. Applied and Environmental Microbiology, 66(8), 3624-3627.

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Prostate Cancer Cell Line Characterization

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Cell lines LNCaP (LNCaP-FGC, RRID:CVCL_1379), CWR22Rv1 (22Rv1, RRID:CVCL_1045), VCaP (RRID:CVCL_2235), DU145 (RRID:CVCL_0105), PC3 (RRID:CVCL_0035) and HEK293T (293T, RRID:CVCL_0063) were purchased from ATCC, PNT2 was purchased from Sigma–Aldrich (Cat# 95012613, RRID:CVCL_2164). All cell lines were cultured under conditions as recommended. C4-2B and C4-2B/Enz^R cells were generous gifts from Dr. Allen C. Gao at UC Davis. Parental C4-2B cells were cultured in the RPMI-1640 medium supplemented with 10% FBS, while C4-2B/Enz^R cells were maintained in the RPMI-1640 complete medium containing 10 μM enzalutamide. All cell lines were recently validated with STR analysis, and confirmed to be mycoplasma-free by Mycoplasma PCR Detection Kit (GeneCopoeia, Rockville, MD USA). Enzalutamide (Enz) was purchased from Selleck Chemicals (Houston, TX, USA).

Hung C.L., Liu H.H., Fu C.W., Yeh H.H., Hu T.L., Kuo Z.K., Lin Y.C., Jhang M.R., Hwang C.S., Hsu H.C., Kung H.J, & Wang L.Y. (2023). Targeting androgen receptor and the variants by an orally bioavailable Proteolysis Targeting Chimeras compound in castration resistant prostate cancer. eBioMedicine, 90, 104500.

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