Nrstar human snorna pcr array

Three pair-matched surgically resected tissues were used to obtain total RNA. OD260/280 measurements on the NanoDrop® ND-1000 (NanoDrop, US) were used to evaluate the purity and concentration of RNA. Denaturing agarose gel electrophoresis was used to test RNA integrity. First-strand cDNA was synthesized using the rtStar™ First-Strand Synthesis Kit (Cat# AS-FS-001, Arraystar, US), cDNA was mixed with Arraystar SYBR® Green qPCR Master Mix (ROX+) (AS-MR-006-5, Arraystar), and snoRNA profiling was performed by using nrStar™ Human snoRNA PCR array according to the manufacturer's instructions (Arraystar, Inc, US) on ABI 7900 real-time PCR system (Applied Biosystems, Foster City, CA). The PCR array consisted of 384 primer sets for analyzing small nucleolar RNAs.

SnoRNAs expression analysis was performed by Arraystar company using the nrStar™ Human snoRNA PCR Array which contains 359 snoRNAs, 7 snoRNA target snRNAs, and 4 snoRNP complex members. 2–5 µg of total RNA from MCF10A DKC1 OE/CTRL were shipped to Arraystar Inc. Experiment and data analyses were performed by Arraystar Inc (Rockville, MD, USA).