2 propanol

Dopamine hydrochloride, ammonium hydroxide, phosphate buffered saline (PBS, 0.01 M, pH 7.4), DMEM medium, Fetal Bovine Serum (FBS), MTT reagent, L-glutathione reduced (GSH) and deferoxamine mesilate (DFO) were provided by Sigma-Aldrich. Penicillin-streptomycin (5000 U/mL) was provided by Thermo Fisher Scientific. Iron (III) chloride anhydrous (FeCl 3 , 97 %), sodium acetate anhydrous, 1-propanol (extra pure), 2-propanol (99.5 %) and 2-methyl-2-propanol (99.5 %) were provided by Panreac. Absolute ethanol (99.5 %) and methanol (99.5 %) were supplied by VWR Chemicals.

Fresh beef and pork meat (loin) and pork back fat were obtained from a local meat market. Sunflower oil (Urzante S.L, Navarra, Spain) was obtained in a local market.
Carrageenan (kappa-carrageenan) was kindly donated by Cargill (San Sebastián, Spain).
Cholesterol, 5α-cholestane, thiobarbituric acid, fatty acid methyl esters and Polysorbate 80 were purchased from Sigma-Aldrich Chemical (Steinheim, Germany). 19hydroxycholesterol was obtained from Steraloids (Wilton, NH, USA). Tri-sil® reagent was obtained from Pierce (Rockford, IL, USA). Acetone, chloroform, ethyl acetate, methanol, hexane, 2-propanol, hydrochloric acid, cyclohexanone, trichloroacetic acid, potassium chloride, potassium hydroxide, anhydrous sodium sulphate and sodium phosphate were obtained from Panreac (Barcelona, Spain). hexane for gas chromatography, dichloromethane for gas chromatography and boron trifluoride/methanol were from Merck (Whitehouse Station, NJ, USA). Strata NH 2 (55 µm, 70 A) 500 mg/3 mL Solid Phase Extraction cartridges were obtained from Phenomenex (Torrance, CA, USA).

Cholesterol, stigmasterol, 5α-cholestane, heptadecanoic acid, α-tocopherol, tocopherol acetate, ammonium thyocianate and thiobarbituric acid were purchased from Sigma-Aldrich Chemical (Steinheim, Germany). 19-hydroxyCholesterol was obtained from Steraloids (Wilton, NH, USA). Tri-sil ® reagent was obtained from Thermo-Scientific (Rockford, IL, USA). Hexane, heptane, acetone, chloroform, ethyl acetate, butanol, methanol, 2-propanol, hydrochloric acid, cyclohexanone, trichloroacetic acid, potassium chloride, potassium hydroxide, ammonium iron (II) sulphate, barium chloride, anhydrous sodium sulphate and sodium phosphate were obtained from Panreac (Barcelona, Spain). Strata NH 2 (55 µm, 70 A) 500 mg / 3 mL Solid Phase Extraction cartridges were obtained from Phenomenex (Torrance, USA). Sunflower oil was purchased in a major local distributor.