Milliplex map human angiogenesis growth factor magnetic bead panel 1

Conditioned media was removed from microchannels at the end of the culture period and stored at −80 °C prior to analysis. Soluble factor levels (presented in Figure 2) were analyzed with Milliplex Map Human Angiogenesis/Growth Factor Magnetic Bead Panel 1 and Milliplex Map Human MMP Panel 1 Magnetic Bead Kits (Millipore). Specifically, media from four replicate channels was collected and mixed together as one sample. Samples were analyzed from three independent experiments (Figure 2B–G) or four independent experiments (Figure 2A). Data were analyzed using Milliplex Analyst v5.1 using a four parameter logistic curve. Factors reported in Figure 2 showed quality controls within the expected range (as indicated by the manufacturer) and levels of soluble factors measured were within the quantifiable region of the standard curve (as determined using Milliplex Analyst v5.1 software); other factors from the panel that did not meet these criteria were not reported.