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Fluo 4 calcium probe

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Fluo-4 is a calcium-sensitive fluorescent probe used for measuring intracellular calcium levels in cells. It is a cell-permeant, acetoxymethyl (AM) ester derivative of the fluorescent dye, Fluo-4. When bound to calcium, Fluo-4 exhibits an increase in fluorescence intensity, allowing for the detection and quantification of calcium dynamics within living cells.

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Lab products found in correlation

Phenol, by Merck Group (2 mentions) Trifluoroacetic acid, by Merck Group (2 mentions) Lysotracker, by Thermo Fisher Scientific (2 mentions) Methyl tert butyl ether (mtbe), by Merck Group (2 mentions) Dichloromethane, by Merck Group (2 mentions) Dimethylformamide, by Merck Group (2 mentions) Iodine, by Merck Group (2 mentions) Bacmam 2, by Thermo Fisher Scientific (2 mentions) Bodipy tr ceramide, by Thermo Fisher Scientific (2 mentions) N n diisopropylethylamine, by Merck Group (2 mentions) Acetic anhydride, by Merck Group (2 mentions) Fmoc lys boc wang resin, by AAPPTec (2 mentions) Q3 system, by Merck Group (2 mentions) N n diisopropylcarbodiimide, by Merck Group (2 mentions) Wang resin fmoc tyr tbu wang resin, by AAPPTec (2 mentions) Celllight peroxisome gfp, by Thermo Fisher Scientific (2 mentions) Chlorpromazine hydrochloride, by Merck Group (2 mentions) Hydroxybenzotriazole, by Merck Group (2 mentions) O 7 azabenzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hatu, by Merck Group (2 mentions) Vectacell rhodamine 123, by Vector Laboratories (2 mentions)

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Fluo-4 (326 citations)

2 protocols using fluo 4 calcium probe

1

Peptide Synthesis and Cellular Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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2

Peptide Synthesis and Cellular Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the fluorenylmethyloxycarbonyl (Fmoc) amino acids, Wang resin Fmoc-Tyr(tBu)-Wang resin and Fmoc-Lys(Boc)-Wang Resin were purchased from AAPPTec (Louisville, KY, USA). Dichloromethane (DCM), acetic acid, acetic anhydride, phenol, hydroxybenzotriazole (HOBt), N,N-diisopropylethylamine (DIEA), N-trityl-1,2-ethanediamine, phenol, thioanisol, dimethylformamide (DMF), N,N’-diisopropylcarbodiimide (DIC), trifluoroacetic acid (TFA), iodine, methyl tert-butyl ether (MTBE) and O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For a full list of chemicals used and their details, please refer to Supplementary Table 1. Water was obtained from a Millipore Q3 system. Lysotracker, BODIPY TR Ceramide, CellLight Peroxisome-GFP, BacMam 2.0, and Fluo-4 calcium probe were purchased from Thermo Fisher Scientific (Waltham, MA). Chlorpromazine hydrochloride, filipin complex, and amiloride hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Reagents for organelle staining included VectaCell Rhodamine 123 (Vector Laboratories, Burlingame, CA, USA).
Shen D., Xu B., Liang K., Tang R., Sudlow G.P., Egbulefu C., Guo K., Som A., Gilson R., Maji D., Mondal S., Habimana-Griffin L., Akers W., Li S., Liu Y., Bloch S., Kurkure S., Nussinov Z., Seidel A., Tsen S.W, & Achilefu S. (2020). Selective imaging of solid tumours via the calcium-dependent high-affinity binding of a cyclic octapeptide to phosphorylated Annexin A2. Nature biomedical engineering, 4(3), 298-313.
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