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2 protocols using na2hpo4 12 h2o

1

Development of a Cholinesterase Inhibitor Assay

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Kolliphor® HS 15 (macrogol 15 hydroxystearate) was a gift from BASF (Ludwigshafen, ermany). Labrafac® WL 1349 and Transcutol® HP were kindly provided by Gattefossé S.A. (Saint-Priest, France). Tegaserod was purchased from Synthenova SAS (Hérouville-Saint-Clair, France). The peptide-22 was synthesized by the peptide team of the Institut des Biomolécules Max Mousseron (IBMM, Montpellier, France) according to Malcor et al. [39 (link)]. Acetylthiocholine iodide (ATC), butyrylthiocholine iodide and 5,5-dithiobis-(2-nitrobenzoic) acid (DTNB), human acetylcholinesterase (AChE), equine butyrylcholinesterase (BuChE), donepezil, Triton® X-100 (octylphenol PEG-10 ether), KH2PO4, KCl and NaH2PO4·H2O were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Tacrine was provided from Tocris (Lille, France). Prisma Buffer HT pH 7,4, sink brain buffer, corticosterone and theophylline was obtained from pION (Billerica, Etats-Unis, MA, USA). Methanol, acetonitrile, acetone, water of HPLC grade were purchased from Carlo Erba Reagents (Val de Reuil, France). Formic acid, Na2HPO4·12 H2O, Tris hydrochloride, HEPES and DMSO were obtained from Fisher Scientific (Illkirch, France). NaCl was provided by Acros Organics (Geel, Belgium).
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2

Curcumin and 5-FU Delivery Polymer

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® a co-polymer of polyvinyl caprolactame-polyvinyl acetate-polyethylene was donated by BASF (Ludwigshafen, Germany). Curcumin (CRC, >94%), 5-fluorouracil (5-FU, >99%), cis-platin (CPT, >99%)) and sodium fluorescein (FlNa) were purchased from Sigma-Aldrich, UK. Padgett Dermatome and dermatome blades were purchased from Integra Life TM Sciences Corporation (PA, USA), KH2PO4 , Na 2 HPO 4 .12H 2 O, NaCl, KCl, NaOH, HCl, acetonitrile, acetic acid, methanol, ethanol, phosphoric acid and hydrochloric acid were purchased from Fisher Scientific (Loughborough, UK).
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