Maltodextrin (MD) ) was provided from Davisco Foods International (Le Sueur, MN, USA), whey protein (WP) was provided from Polmlek (Raciąż, Poland), pectin was provided from CPKelco (Großenbrode, Germany), and corn starch was supplied from Production sector of the Institute for Medicinal Plants Research Dr. Josif Pančić. Gelatin was produced by Aleva, Novi Kneževac. Folin-Ciocalteu reagent, gallic acid, orthophosphoric acid, and sodium carbonate were purchased from Sigma-Aldrich Chemie GmbH (Munich, Germany). Ultra-pure water was prepared using a Milli-Q purification system (Millipore, France), and HPLC-grade acetonitrile was obtained from Merck (Darmstadt, Germany). Standards swertiamarin, sweroside, and gentiopicroside were purchased from ChromaDex, USA, isoorientin and isovitexin were from Extrasynthese (Cedex, France), and isogentisin was purchased from Phytolab (Germany).
Gentiopicroside
Manufactured by ChromaDex
Sourced in United States
Gentiopicroside is a pure compound extracted from the roots of Gentiana plants. It is a colorless, crystalline substance that can be used as a reference standard in analytical testing procedures.
Automatically generated - may contain errors
Lab products found in correlation
Orthophosphoric acid, by Merck Group (3 mentions)
Isogentisin, by Phytolab (2 mentions)
Milli q purification system, by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Hplc grade acetonitrile, by Merck Group (2 mentions)
Isoorientin, by Extrasynthese (1 mentions)
Swertiamarin, by ChromaDex (1 mentions)
Sweroside, by ChromaDex (1 mentions)
Isovitexin, by Extrasynthese (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Gallic acid, by Extrasynthese (1 mentions)
Hp β cd, by Thermo Fisher Scientific (1 mentions)
Folin ciocalteu s phenol reagent, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Methocel e5 lv, by Dow (1 mentions)
Pd98059, by New England Biolabs (1 mentions)
Sb203580, by Merck Group (1 mentions)
4 protocols using gentiopicroside
1
Characterization of Medicinal Plant Compounds
2
Phytochemical Analysis of Medicinal Plants
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Ultrapure water was prepared using a Milli-Q water purification system (Millipore, France). HPβCD (97%) was obtained from Acros Organics. Folin–Ciocalteu′s phenol reagent, sodium carbonate, orthophosphoric acid, and methanol were purchased from Sigma–Aldrich Chemie GmbH (Munich, Germany), while HPLC-grade acetonitrile was purchased from Merck (Darmstadt, Germany). Gallic acid (Extrasynthese, Genay, France), gentiopicroside (ChromaDex, Los Angeles, CA, USA), and isogentisin (Phytolab, Vestenbergsgreuth, Germany) were used as standards.
3
Gentiopicroside Formulation and Analysis
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Excipients used for formulation were hydroxypropyl methylcellulose (HPMC, Methocel ® E5 LV, Dow ® , USA), hydrophilic polymer used as a matrix forming excipient, lactose monohydrate was used as a diluent, citric acid and sodium bicarbonate as an effervescent components, while glyceryl behenate (Compritol ® 888 ATO, Gattefrosse, Saint-Priest, Cedex, France) and stearoyl macrogol-32 glycerides (Gelucire ® 50/13, Gattefrosse, Saint-Priest, Cedex, France) were used as meltable binders. Ethanol 96 % (v/v) (Honeywell Riedel de Haën, Seelze, Germany), orthophosphoric acid (Sigma-Aldrich Chemie GmbH, Münich, Germany), acetonitrile (Merck, Germany) was HPLC grade and ultra-pure water was prepared using a Milli-Q purification system (Millipore, France). Gentiopicroside (ChromaDex, USA) standard was used. All excipients, reagents and standards used were of analytical grade.
4
Immunohistochemical Analysis of CerS3 Expression
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The polyclonal rabbit-anti-human CerS3 antibody was from Antikörper-online (Aachen, Germany; 1:50). The secondary antibodies were the swine anti-rabbit-FITC antibody (Dako, Hamburg, Germany; 1:30) and the donkey anti-rabbit-Alexa-Fluor 555 antibody (Thermo Fisher Scientific, Darmstadt, Germany). DAPI was from Sigma-Aldrich GmbH (Taufkirchen, Germany). The following specific inhibitors were used: PPARγ antagonist (GW9962, Enzo, Lörrach, Germany), PI3K inhibitor (LY294002; Sigma-Aldrich GmbH), ERK inhibitor (PD98059, New England Biolabs GmbH, Frankfurt, Germany), p38 MAPK inhibitor (SB203580, Sigma-Aldrich GmbH) and NFκB inhibitor (GIV 3727, Merck Millipore, Darmstadt, Germany). Methanolic sodium hydroxide solution, hexane standards, loganic acid, and loganin were from Carl Roth GmbH (Karlsruhe, Germany). Boron trifluoride–methanol complex was from Merck KGaA (Darmstadt, Germany), and standard methyl heptadecanoate was from Sigma-Aldrich GmbH. Orto-phosphoric acid, 85%, Ph. Eur. p.a. grade was supplied by VWR. Amarogentin and gentiopicroside were purchased from Chromadex Inc. (Santa Ana, CA, USA).
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