Alexa fluor 488 labelled goat anti rabbit igg h l secondary antibody

Lico A (purity > 98%) was acquired from Beijing Solarbio Science &Technology Co, Ltd (Beijing, China). Cell‐Counting Kit‐8 (CCK‐8) assays were purchased from Dojindo (Kumamo, Japan). Primary antibodies directed against aggrecan, collagen‐II, IκBα, NLRP3,ASC,pro‐caspase 1 and Lamin B were obtained from Abcam (Cambridge, MA, USA). Type II collagenase, LPS and dimethylsulphoxide (DMSO) were acquired from Sigma Chemical Co. (St. Louis, MO, USA). Primary antibodies against Gasdermin D (GSDMD), cleaved‐GSDMD, Nrf2, HO‐1, cleaved caspase‐1, glyceraldehyde‐3‐phosphate dehydrogenase (GADPH) were acquired from Cell Signaling Technology (MA, USA). Alexa Fluor®594‐labelled, Alexa Fluor®488‐labelled goat anti‐rabbit IgG (H + L) secondary antibody and Goat anti‐rabbit IgG‐HRP were was obtained from Jackson ImmunoResearch (West Grove, PA, USA). Enzyme‐linked immunosorbent assay (ELISA) kits were obtained from R&D systems (Minneapolis, MN, USA). Foetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM)/Ham's F12 medium were purchased from Gibco (Grand Island, NY, USA).

Procyanidin B3 (purity 95%) was obtained from BOC sciences. Other reagents used were as follows: primary antibodies for p65, IκBα and COX‐2 (CST), GADPH, TRAF6, MyD88, ADAMTS5, Lamin B, collagen Ⅱ and TLR4 (Abcam). Anti‐IRAK1, goat antimouse and goat anti‐rabbit IgG‐HRP were bought from Bioworld. Recombined human TLR4 and MD2 protein (rhTLR4 and rhMD2) were bought from R&D Systems. Anti‐MD‐2 and Biotin‐labelled LPS (Biotin‐LPS) were obtained from eBioscience. Type II collagenase, fast green and Safranin‐O were bought from (Sigma‐Aldrich) and Alexa Fluor^®488 labelled Goat Anti‐Rabbit IgG (H + L) secondary antibody (Jackson ImmunoResearch).