Ritodrine

Isoproterenol, forskolin, ICI 118-551 (ICI), epinephrine, norepinephrine, salbutamol, salmeterol, ritodrine, fenoterol, alprenolol, glucagon, oxyntomodulin, GLP-1-(7-36), rolipram, zaprinast, caffeine, HEPES, IBMX, magnesium and calcium chloride, bovine serum albumin (BSA), and Whatman glass microfiber filters (grade GF/B) were purchased from Sigma-Aldrich (Broendby, Denmark). Dulbecco's modified Eagle's medium (DMEM), Hank's balanced salt solution (HBSS), fetal bovine serum (FBS), zeocin, geneticin G418, and penicillin/streptomycin were purchased from Invitrogen (Naerum, Denmark). Black 384-well plates were purchased from Greiner Bio-One (Kremsmünster, Austria). [ 3 H] dihydroalprenolol and EnVision Xcite multilabel plate reader were from PerkinElmer (Waltham, MA).

The GPCR compound library, VX-809 and VX-770 were purchased from SelleckChem (Houston, TX, USA). Carvedilol, forskolin, salbutamol, salmeterol, terbutaline, epinephrine, ritodrine, dimethylsulphoxide (DMSO), N-acetylcysteine, nicotinamide and SB202190 were purchased from Sigma (St Louis, MO, USA). Formoterol was purchased from Santa Cruz Biotechnologies (Dallas, TX, USA). CFTR inh -172 was obtained from CFF Therapeutics (Chicago, IL, USA). Matrigel was purchased from BD (Franklin Lakes, NJ, USA). Calcein AM, supplements N-2 and B-27, Glutamax, advanced Dulbecco's modified Eagle medium/Ham's F-12 (DMEM/F-12), penicillin/streptomycin, HEPES and murine epidermal growth factor (mEGF) were purchased from Life Technologies (Bleiswijk, The Netherlands). A83-01 was purchased from Tocris (Abingdon, UK). TOPflash and FOPflash were purchased from Millipore (Amsterdam, The Netherlands).