C57bl 6 tg cag egfp mice

Manufactured by Japan SLC

Sourced in Japan

The C57BL/6-Tg (CAG-EGFP) mice are a transgenic mouse strain that expresses enhanced green fluorescent protein (EGFP) under the control of the cytomegalovirus (CMV) early enhancer/chicken beta-actin (CAG) promoter. This allows for the visualization of cells and tissues expressing the EGFP reporter gene.

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Lab products found in correlation

C57bl 6 mice, by Japan SLC (2 mentions) Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions) Tryple select, by Thermo Fisher Scientific (1 mentions) Collagenase 2, by Merck Group (1 mentions) 70 μm nylon mesh, by BD (1 mentions) Antibiotic antimycotic solution, by Merck Group (1 mentions) Penicillin streptomycin amphotericin b, by Merck Group (1 mentions) C57bl 6 wt mice, by CLEA Japan (1 mentions) C57bl 6j, by Japan SLC (1 mentions) Balb c nu nu, by Japan SLC (1 mentions) Balb c, by Japan SLC (1 mentions) Lysm cre, by Jackson ImmunoResearch (1 mentions) Rosa26 eyfp, by Jackson ImmunoResearch (1 mentions) Thbs1, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Japan SLC (1 mentions) P53 mice, by RIKEN BioResource Center (1 mentions) Rosa26 creert2 mice, by Taconic Biosciences (1 mentions) Rosa26 lsl cas9 knockin mice, by Jackson ImmunoResearch (1 mentions) Nsg mice, by Charles River Laboratories (1 mentions) C57bl 6j mice, by Japan SLC (1 mentions)

Spelling variants of this product

C57BL/6 mice (249 citations) C57BL/6 (47 citations) C57BL/6-Tg (CAG-EGFP) (10 citations) C57BL/6-Tg (CAG-EGFP) male mice (2 citations)

9 protocols using c57bl 6 tg cag egfp mice

Generation of CLEC-2-deficient Chimeric Mice

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Because CLEC-2-null mice are embryonic/neonatal lethal, CLEC-2-deficient irradiated chimeric mice were generated as described previously[6 (link)]. Briefly, irradiated adult C57BL/6 male mice were rescued by intravenous injection of 1 × 10⁶ fetal liver cells from CLEC-2^−/− (CLEC-2-deficient chimera) or CLEC-2^+/+ embryos (wild-type (WT) chimera). We confirmed complete bone marrow engraftment in all the CLEC-2-deficinet chimeras by flow cytometry. C57Bl/6-Tg (CAG-EGFP) mice were purchased from Japan SLC Inc. These mice were crossed with CLEC-2^+/− mice to generate CAG-EGFP;CLEC-2^+/− offspring. CAG-EGFP;CLEC-2^+/− mice were crossed to obtain CAG-EGFP;CLEC-2^+/+ and CAG-EGFP;CLEC-2^−/− embryos. Where indicated, these GFP-positive embryos were used to generate GFP-positive WT chimeras and GFP-positive CLEC-2-deficient chimeras.
Apolipoprotein (Apo) E-/- mice were purchased from Jackson Laboratories and fed a high fat diet for 8 weeks. This study was approved by the Animal Care and Use Committee at the University of Yamanashi.

Inoue O., Hokamura K., Shirai T., Osada M., Tsukiji N., Hatakeyama K., Umemura K., Asada Y., Suzuki-Inoue K, & Ozaki Y. (2015). Vascular Smooth Muscle Cells Stimulate Platelets and Facilitate Thrombus Formation through Platelet CLEC-2: Implications in Atherothrombosis. PLoS ONE, 10(9), e0139357.

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Generation and Characterization of MFG-E8 KO Mice

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MFG-E8 KO C57BL/6 mice were generated, and genotyped as described previously (24 (link), 29 (link), 31 (link)). MFG-E8 KO mice were generated by interbreeding homozygous animals carrying the targeted MFG-E8 allele. Interbreeding homozygous C57BL/6 mice and C57BL/6-Tg (CAG-EGFP) mice were purchased from Japan SLC. Eight- to twelve-week-old mice were used for all experiments.

Yamada K., Uchiyama A., Uehara A., Perera B., Ogino S., Yokoyama Y., Takeuchi Y., Udey M.C., Ishikawa O, & Motegi S.I. (2016). MFG-E8 drives melanoma growth by stimulating mesenchymal stromal cell-induced angiogenesis and M2 polarization of tumor-associated macrophages. Cancer research, 76(14), 4283-4292.

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Isolation of Adipose-Derived Stem Cells from Transgenic Mice

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C57BL/6-Tg(CAG-EGFP) mice were purchased from Japan SLC Inc. (Shizuoka, Japan). ADSCs were isolated from the subcutaneous adipose tissue of 8- to 12-week-old C57BL/6-Tg(CAG-EGFP) mice as described previously [27 (link)]. Briefly, we obtained the adipose tissue from the subcutaneous fat pad around the ilium of the mouse and incubated the tissue in phosphate-buffered saline (PBS) containing antibiotic-antimycotic solution (Sigma-Aldrich, St. Louis, MO). We minced the fat pad into fine pieces and then incubated the pieces in Dulbecco's modified Eagle's medium (DMEM) (Sigma-Aldrich) containing 1 mg/ml collagenase II (Sigma-Aldrich) and 1% Penicillin-Streptomycin-Amphotericin B (Sigma-Aldrich) at 37°C for 30 min. The digested tissue was filtered through a sterile 70 μm nylon mesh (BD FALCON, Corning, NY), the filtrate was centrifuged at 1800 rpm for 5 min, and the supernatant was discarded. We resuspended the pellet in a 10 ml culture medium. This process was repeated twice. We seeded ADSCs onto culture dishes with growth medium (DMEM containing 10% fetal bovine serum (FBS) (Sigma-Aldrich) and 1% Penicillin-Streptomycin-Amphotericin B suspension). We dissociated the cells with TrypLE™ Select (Thermo Fisher Scientific, Waltham, MA).

Fukui E., Funaki S., Kimura K., Momozane T., Kimura A., Chijimatsu R., Kanzaki R., Kanou T., Ose N., Minami M., Miyagawa S., Sawa Y., Okumura M, & Shintani Y. (2019). Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema in Mice. Stem Cells International, 2019, 5179172.

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Transgenic Mouse Strains for Immunology Research

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WT C57BL/6 mice were obtained from CLEA Japan. GM-CSF–KO (B6.129S-Csf2^tm1Mlg/J) mice (Dranoff et al., 1994 (link)) were obtained from The Jackson Laboratory. C57BL/6-Tg (CAG-EGFP) mice (Okabe et al., 1997 (link)) were obtained from Japan SLC. MyD88-KO (B6.129-Myd88^tm1Aki/Obs) mice (Adachi et al., 1998 (link)) were obtained from Oriental Bio Service. CXCL12-GFP mice were developed as previously reported (Sugiyama et al., 2006 (link)). IL-33^GFP/+ and IL-33^GFP/GFP (IL-33–deficient) mice (Oboki et al., 2010 (link)) were kindly provided by S. Nakae (University of Tokyo, Tokyo, Japan) and H. Kiyonari (RIKEN, Kobe, Japan; accession no. CDB0631K; http://www.clst.riken.jp/arg/mutant%20mice%20list.html). These mouse lines were maintained on a C57BL/6 background. All mice used in this study were 8–14 wk old. Animal studies were performed with the approval of the Institutional Review Board of Osaka University.

Sudo T., Motomura Y., Okuzaki D., Hasegawa T., Yokota T., Kikuta J., Ao T., Mizuno H., Matsui T., Motooka D., Yoshizawa R., Nagasawa T., Kanakura Y., Moro K, & Ishii M. (2021). Group 2 innate lymphoid cells support hematopoietic recovery under stress conditions. The Journal of Experimental Medicine, 218(5), e20200817.

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Islet Transplantation and Neovascularization in Mice

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Male C57BL/6J, Balb/c, and Balb/c nu/nu mice were purchased from Japan SLC Inc., (Shizuoka, Japan). For all experiments, recipients were 8–10 wk old, and donors were 10–14 wk old. The recipient and follow-up mice were housed separately. All animals had free access to a standard diet and water. The experiments were approved by the local ethics committee (approved protocol ID: 2018 Medical-Animal-129) and performed in accordance with national and institutional regulations. Animals were maintained in a pathogen-free environment.
All surgeries were performed under anesthesia, and all efforts were made to minimize suffering. Male C57BL/6-Tg (CAG-EGFP) mice (9–12 wk old; Japan SLC Inc.) were used as islet donors for neovascularization experiments.

Fathi I., Nishimura R., Imura T., Inagaki A., Kanai N., Ushiyama A., Kikuchi M., Maekawa M., Yamaguchi H, & Goto M. (2021). KRP-203 Is a Desirable Immunomodulator for Islet Allotransplantation. Transplantation, 106(5), 963-972.

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Genetically Modified Mouse Models

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Thbs1-deficient (Thbs1^-/- (#006141)), LysM-Cre (#004781) and Rosa26-EYFP (#006148) mice were purchased from Jackson Laboratories. C57BL/6 mice, GFP transgenic mice (C57BL/6-Tg (CAG-EGFP)) mice were purchased from Japan SLC,Inc. Systemic CD47-deficient (CD47^-/-) (CD47^f/f;E2A-Cre) mice were generated by crossing CD47^f/f mice with E2A-Cre mice^{49 (link)}. Thbs1^f/f mice were generated as previously described^{39 (link)}. CD36-deficient (CD36^-/-) mice were kindly provided by Dr. Freeman (Harvard Medical School). All mouse strains were generated in a C57BL/6 background and were born and maintained under pathogen-free conditions. These mice were maintained in 14 hr light/10 h dark cycle, and the housing temperature and humidity were maintained were 24 °C and 50%, respectively. Animal handling and experimental procedures conformed to institutional guidelines and were approved by the animal research committee of Kyoto University (Kyoto, Japan) and performed in accordance with Japanese government regulations. 8–10 weeks old male mice were used in all experiments.

Omatsu M., Nakanishi Y., Iwane K., Aoyama N., Duran A., Muta Y., Martinez-Ordoñez A., Han Q., Agatsuma N., Mizukoshi K., Kawai M., Yamakawa G., Namikawa M., Hamada K., Fukunaga Y., Utsumi T., Sono M., Masuda T., Hata A., Araki O., Nagao M., Yoshikawa T., Ogawa S., Hiramatsu Y., Tsuda M., Maruno T., Kogame T., Kasashima H., Kakiuchi N., Nakagawa M.M., Kawada K., Yashiro M., Maeda K., Saito Y., Matozaki T., Fukuda A., Kabashima K., Obama K., Ogawa S., Sheibani N., Diaz-Meco M.T., Moscat J, & Seno H. (2023). THBS1-producing tumor-infiltrating monocyte-like cells contribute to immunosuppression and metastasis in colorectal cancer. Nature Communications, 14, 5534.

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Mouse Experimental Protocols for GFP Expression

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C57BL/6 and C57BL/6-Tg (CAG-EGFP) mice (6 to 12 weeks old) were purchased from Japan SLC (Shizuoka, Japan). All experimental procedures in this study were approved by the Institutional Animal Care and Use Committee of Osaka University. Mice were handled and maintained according to the Osaka University guidelines for animal experimentation.

Iba T., Naito H., Shimizu S., Rahmawati F.N., Wakabayashi T, & Takakura N. (2019). Isolation of tissue-resident endothelial stem cells and their use in regenerative medicine. Inflammation and Regeneration, 39, 9.

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Genetic Mouse Models for Research

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C57BL/6J mice were purchased from Japan SLC. Ly5.1 mice were maintained in IRCMS, Kumamoto University. NSG mice were purchased from Charles River Laboratories Japan. NSGS mice for PDX experiments were purchased from the Jackson Laboratory. p53^−/− mice, in which 5′ part of exon 2 including translation initiation site of Trp53 gene was replaced with Neomycin resistance gene, were provided from the RIKEN BioResource Center (Ibaragi, Japan)^{42 (link)}. Hif1α^flox/flox mice^{43 (link)} were provided from Dr. Randall S. Johnson, and were crossed with Rosa26-Cre-ERT2 mice (Taconic). Rosa26-LSL-Cas9 knockin mice were purchased from Jackson Laboratory^{44 (link)}. C57BL/6-Tg(CAG-EGFP) mice were purchased from Japan SLC. The primer sequences for genotyping are provided in Supplementary Table 2. All animal studies were approved by the Animal Care Committee of the Institute of Medical Science at the University of Tokyo (approval number: PA13-19, PA15-100, PA17-75), and were conducted in accordance with the Regulation on Animal Experimentation at University of Tokyo based on International Guiding Principles for Biomedical Research Involving Animals.

Hayashi Y., Goyama S., Liu X., Tamura M., Asada S., Tanaka Y., Fukuyama T., Wunderlich M., O’Brien E., Mizukawa B., Yamazaki S., Matsumoto A., Yamasaki S., Shibata T., Matsuda K., Sashida G., Takizawa H, & Kitamura T. (2019). Antitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia. Nature Communications, 10, 4869.

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Hepatocyte Transplantation in Mouse Models

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All animal studies were referred to the ARRIVE guidelines and were
followed the institutional guidelines outlined by the Institutional Animal Care
and Use Committees at Nara Medical University, Tokyo Women's Medical
University, and University of Pittsburgh. Human α1-antitrypsin (hA1AT)
promoter/hA1AT-Tg mice (FVB/N-Tg(hA1AT);
8–20 wks old; kindly provided by Dr. G. L. Bumgardner), C57BL/6NCrSlc
mice (7–9 wks old; Japan SLC, Shizuoka, Japan), and
C57BL/6-Tg(UBC-GFP)30Scha/J mice (5–8 wks old; the
Jackson Laboratory, Bar Harbor, ME) were used to obtain hepatocytes for
transplantation. FVB/N mice (7–9 wks old; CLEA Japan, Tokyo, Japan),
C57BL/6-Tg(CAG-EGFP) mice (7–9 wks old; Japan SLC),
and Fah^−/− mice backcrossed into the
C57BL/6 mouse background (12 wks old; 129sv; kindly provided by Dr. M. Grompe)
were used as the recipients. Mice were housed in cages within a temperature and
humidity controlled room (22–24°C, 55 ± 10%
humidity) with a 12 h light/12 h dark cycle. All mice were fed a normal chow
diet with ad libitum access to water and food.
For further details, please refer to the Supplementary Materialand CTAT table.

Utoh R., Komori J., Kuge H., Tatsumi K., Yamada M., Hirohashi S., Tsutsumi M., Amanuma T., Yoshioka A., Nakajima Y., Wake K., Okano T., Lagasse E, & Ohashi K. (2017). Adult hepatocytes direct liver organogenesis through non-parenchymal cell recruitment in the kidney. Journal of hepatology, 68(4), 744-753.

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