Four human ESCC cell lines (Eca109, EC9706, KYSE150, KYSE450) and human normal esophageal epithelial cell line (Het-1A) were all procured from the ATCC (Manassas, VA, USA) and propagated at 37 °C in a humidified incubator of 5% CO2. Cell lines were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) with 10% FBS (Gibco) and 1% Pen/Strep mixture.
Eca109
Manufactured by American Type Culture Collection
Sourced in United States
The Eca109 is a laboratory-grade cell culture incubator that provides a stable and controlled environment for the cultivation of cells. It maintains consistent temperature, humidity, and gas composition to support the growth and proliferation of various cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (34 mentions)
Kyse 150, by American Type Culture Collection (29 mentions)
Ec9706, by American Type Culture Collection (25 mentions)
Het 1a, by American Type Culture Collection (17 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (16 mentions)
Kyse450, by American Type Culture Collection (11 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (11 mentions)
Rpmi 1640, by Thermo Fisher Scientific (9 mentions)
Kyse30, by American Type Culture Collection (9 mentions)
Te 10, by American Type Culture Collection (8 mentions)
Streptomycin, by Thermo Fisher Scientific (6 mentions)
Eca 109, by Thermo Fisher Scientific (6 mentions)
Penicillin, by Thermo Fisher Scientific (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Kyse510, by American Type Culture Collection (4 mentions)
Kyse410, by American Type Culture Collection (4 mentions)
Dmem medium, by Thermo Fisher Scientific (3 mentions)
Caes 17, by American Type Culture Collection (3 mentions)
Kyse170, by American Type Culture Collection (3 mentions)
Rs 2000 pro x ray bio irradiator, by Rad Source (3 mentions)
81 protocols using eca109
1
Culturing Human ESCC Cell Lines
2
Esophageal Cancer Cell Line Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEEC and 4 esophageal cancer cell lines—EC9706, Eca109, KYSE-150, and TE-102—were purchased from ATCC (Manassas, VA, USA). After thawing, the cells were cultured with Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (26140079; Gibco, Carlsbad, CA, USA) and 1% penicillin-streptomycin at 37°C with 5% CO2. The medium was replaced every day. When cell confluence reached 60%–70%, cell transfection was performed based on the instructions of the Lipofectamine 2000 kit (Thermo Fisher Scientific, Waltham, MA, USA). The plasmids, including sh-NC, sh-KDM5B, sh-SOX17, oe-NC, oe-KDM5B, oe-SOX17, and oe-GREB1, as well as the mimic NC, let-7i mimic, inhibitor-NC, and let-7i inhibitor were all purchased from Sino Biological (Beijing, China). After 48 h of transfection, the cells were employed for subsequent experiments.
3
Overexpression and Silencing of ARHI in ESCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human ESCC cell line ECA109 (purchased from ATCC, Manassas, VA, USA) was cultured in RPMI-1640 (GIBCO, Waltham, MA, USA; 1.5 g/L NaHCO3, 2.5 g/L glucose, 0.11 g/L sodium pyruvate), with 10% fetal calf serum, at 37°C and 5% CO2. PCMV-ARHI-AC-GFP and PCMV-AC-GFP were purchased from OriGene (Rockville, MD, USA). The ARHI plasmid PCMV-ARHI-AC-GFP was transfected into ECA109 cells; ARHI was overexpressed in vitro and verified by western blotting. The empty plasmid PCMV-AC-GFP (OriGene) was used as a negative control. ARHI-targeted small interfering RNA (siRNA) was purchased from GenePharma (Shanghai, China), the sequence of target was: CUGCUUGACAAGUGCAUAATT. Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) was used for transfection according to the manufacturer’s instructions. After 48 h, the transfected cells were harvested and used for further experiments.
4
Culturing Human Esophageal Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normal esophageal epithelial cells, human esophageal carcinoma cell line (TE-1) and three different human esophageal squamous carcinoma cell lines (KYSE150, ECA-109, EC9706) were purchased from ATCC (Rockville, MD, USA). These cells were cultured in DMEM (Gibco, Scoresby, Australia) containing with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA) and passaged using 0.25% Trypsin (Thermo Fisher Scientific).
5
Culture of EC and THP-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EC cells (Eca-109 and TE-9) and THP-1 cells were purchased from ATCC. The cells were cultured in DMEM (Invitrogen, Thermo Fisher Scientific, Inc., Shanghai, China), supplemented with 10% FBS, 1% streptomycin and penicillin (Invitrogen) in 5% CO2 at 37˚C.
6
Culturing ESCC and Esophageal Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human ESCC cells (Kyse-150, TE-10, Eca109) and human esophageal epithelial cell (HET-1A), from ATCC (Rockville, Maryland, MD, USA) are grown at 37 °C with 5% CO2. RPMI 1640 medium (Gibco, Grand Island, NY, USA) was used for cell culture with 10% FBS (Gibco) and 1% antibiotics. The medium was changed every three days.
7
Regulation of PLP2 by circRNA_2646 in ESCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human normal esophageal epithelial cells HEEC and ESCC cell lines (KYSE150, Eca109, TE-1, and Eca9706) were obtained from ATCC, USA. The cell lines were maintained in a low-glucose DMEM culture medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin with 5% CO2 under humidified condition. The pCD5-circRNA_2646, pcDNA3.1-PLP2, their negative control vectors, and the renilla luciferase reporter vector psiCHECK2-circRNA_2646 with wild (WT) or mutant (MUT) miR-124-binding sites were constructed by GenePharma (Shanghai, China). The oligonucleotide sequences of miR-124 mimics or negative control (NC) were purchased from RiboBio (Guangzhou, China). A reporter plasmid of full-length 3′-UTR (WT or MUT) of PLP2 was constructed by GenePharma (Shanghai, China).
8
ESCC Tumor Tissue and Cell Line Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The paired tumor tissues and adjacent normal tissues (n = 42) were collected from ESCC patients who underwent surgery resection at the Shangqiu first People’s Hospital. This study was approved by the Ethics Committee of Shangqiu first People’s Hospital and informed consents were signed by all patients. The normalized RNA-seq data of Esophageal Carcinoma (ESCA) were downloaded from the TCGA data portal website (https://cancergenome.nih.gov/ ).
Human immortalized esophageal epithelial cell line HET-1A and human ESCC cell lines (ECA109 and EC9706) were purchased from ATCC (Manassas, VA, USA). All cells were cultured in PRMI-1640 medium (Gibco, Rockville, MD, USA) supplemented with 10% FBS (Gibco) at 37 °C with 5% CO2. DDP-resistant variants (ECA109/DDP and EC9706/DDP) of ECA109 and EC9706 cells were established using a repetitive pulsatile treatment with constant concentrations of cisplatin [17 (link)]. The degree of chemotherapy resistance of DDP-resistant variants was evaluated before transfections.
Human immortalized esophageal epithelial cell line HET-1A and human ESCC cell lines (ECA109 and EC9706) were purchased from ATCC (Manassas, VA, USA). All cells were cultured in PRMI-1640 medium (Gibco, Rockville, MD, USA) supplemented with 10% FBS (Gibco) at 37 °C with 5% CO2. DDP-resistant variants (ECA109/DDP and EC9706/DDP) of ECA109 and EC9706 cells were established using a repetitive pulsatile treatment with constant concentrations of cisplatin [17 (link)]. The degree of chemotherapy resistance of DDP-resistant variants was evaluated before transfections.
9
Esophageal Cancer Cell Lines Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
10
Cell Culture Protocols for Esophageal Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, the human EC cell lines (Eca109, KYSE150, EC9706) and normal esophageal epithelial cells (Het-1A) were acquired from the ATCC (Manassas, VA, USA). These cells were cultured in 1640 medium (RPMI; Gibco, CA, USA) and supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco, CA, USA). HEK-293T cells were maintained in DMEM (Gibco, CA, USA) and added the same ingredients as above. These cells were cultured at 37°C under 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!