The rats were divided into the following groups: sham-control (n=5), HS (n=5) and Bcl-xL plus HS (n=5). HS was induced as described above. Visualization and quantification of ROS from the mesenteric post-capillary venules was performed using dihydrorhodamine 123 (DHR 123; 50 mg/kg) using intravital microscopy. Images of the mesenteric venules were obtained before the shock period and at 10, 20, 30, 40, 50 and 60 min into resuscitation. To determine relative expression of ROS, the fluorescent intensity was measured in two areas along the vessel using MetaMorph 4.5/4.6 (Universal Imaging Corp.). Values of DHR 123 fluorescence were expressed as change in intensity over time versus baseline values.
Metamorph 4 5 4
Manufactured by Universal Imaging
MetaMorph 4.5/4.6 is a software application developed for image acquisition, processing, and analysis. It provides a range of tools for managing and manipulating digital images obtained from microscopy and other imaging systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using metamorph 4 5 4
1
Mesenteric ROS Visualization in Shock
2
Visualization of Oxidative Stress in Shock
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