Gelatin peptone

Vegetative isolation was conducted in the potato dextrose agar (PDA) medium. For evaluation of the strains (growth rate and biomass production) in solid media, malt extract-peptone and yeast agar (ME-PYA) and PDA were used. The PDA medium was prepared with 39% w/v potato dextrose agar BD Bioxon^MR; the medium ME-PYA was prepared with 33.6% w/v malt extract agar BD Difco™, 2% w/v yeast extract BD Bioxon^MR and 1% w/v gelatin peptone BD Bioxon^R. For the liquid media, malt extract with peptone and yeast (ME-PY) and potato dextrose (PD) were used. The medium ME-PY was prepared with 33.6% w/v malt extract BD Bacto™, 2% w/v yeast extract BD Bioxon^MR and 1% w/v gelatin peptone BD Bioxon^R. The medium PD was prepared with 39% w/v potato dextrose broth BD Difco™. All culture media were sterilized in an autoclave at 121 °C, 15 Ibs psi for 15 min and 0.05 g of Chloramphenicol (Sigma^®) were added.