Glp 1r antibody

In some PET ex vivo autoradiography experiments, the same sections (n = 10) of the pancreas as used for the autoradiography (n = 24) were stained with GLP1-R antibody (abcam, Cambridge, UK). In some other PET ex vivo autoradiography experiments, two adjacent sections (6 μm) were stained with glucagon (n = 10) and insulin (n = 10) antibodies (abcam, Cambridge, UK). Sections were fixed in pre-cooled (−20 °C) acetone for 10 min. After evaporation of acetone from the tissue sections for 20 min at room temperature, the slides were washed in phosphate buffered saline (PBS) 2 times (5 min each), incubated in 0.3% hydrogen peroxide solution at room temperature for 10 min to block endogenous peroxidase activity, washed in PBS, followed by incubation with the primary GLP-1R (ab39072), insulin (ab63820) or glucagon antibody (ab92517). The slides were washed and incubated in Flex+/HRP (Dako, Glostrup, Denmark) for 15 minutes. After another wash, the slides were developed with DAB+ (Dako, Glostrup, Denmark), counterstained with hematoxylin (Biocare Medical, San Diego, CA, USA), rinsed in water, dehydrated, and finally mounted.

Six‐to‐eight‐week‐old male C57BL/6 mice (18‐22 g) were obtained from the Research Animal Center of Shanxi Medical University with approval of the Shanxi Animal Research Ethics Committee, and the procedures were performed in accordance with ethical guidelines. The approval code is SYXK2015‐0001. The mice were placed in an environment with appropriate temperature and humidity, and food was provided ad libitum during the entire study.
The main reagents for the experiment included Aβ31‐35 (Abcam), D‐Ser2‐oxyntomodulin (provided by Professor Christian Hölscher), Bmal1 antibody (Abcam), Per2 antibody (Santa Cruz Biotechnology), and GLP‐1R antibody (Abcam).