Anti-con, anti-miR-148a, Con-mimic, and miR-148a-mimic were synthesized by RiBoBio Co. Cells were transiently transfected using the Lipofectamine 2000 reagent (Invitrogen, Carlsbad, USA) for 12 h, according to the manufacturer's protocol. For gene recovery assay, after MHCC97H cells were transfected by anti-miR-148a for 12 h, they were cultured in fresh DMEM medium supplemented with 10% FBS (Gibco), 100 U/ml penicillin, and 100 µg/ml streptomycin (Gibco) for another 24 h, followed by transfected with Con-mimic or miR-148a-mimic for 12 h.
Anti mir 148a
Manufactured by RiboBio
Sourced in United States, China
Anti-miR-148a is a laboratory product designed to inhibit the function of miR-148a, a microRNA molecule. It is intended for use in research applications to study the biological role and regulatory functions of miR-148a.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti con, by RiboBio (1 mentions)
Con mimic, by RiboBio (1 mentions)
Mir 148a mimic, by RiboBio (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti mir 148a
1
Transfection of Anti-miR-148a and Mimics
2
Inhibition of miR-148a in Breast Cancer
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Briefly, breast cancer cells were seeded in six-well plates at a density of 1 × 105 per well. After 48 h, anti-miR-negative control and anti-miR-148a (RiBoBio Guangzhou, China) were transfected in cells at 50 nM using Lipofectamine® 2000 (Invitrogen) following the standard protocol. After 12 h of transfection, the culture medium was replaced by fresh DMEM containing 10% FBS (Gibco) for another 24 h before further experiments. The information of miRNA inhibitors used in this study is shown in Additional file 2 : Table S2.
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