IL1-β, IL-6, IL-8, IL-10, TNF-α, and IFN-γ were measured using two multiplexed immunometric assay panels for human cytokines (Luminex® xMAP®, R&D Systems®, R&DSystemsTM, Minneapolis, MN). The antibody-conjugated beads are allowed to react with the sample and a secondary detection antibody to form a capture sandwich immunoassay. After the assay was completed, the assay solution is drawn into the Bio-Plex™ 200 Luminex array reader (BioRAD, Hercules, CA) to quantify the analytes.
Bio plex 200 luminex array reader
Manufactured by Bio-Rad
The Bio-Plex™ 200 Luminex array reader is a multiplex detection system that allows for the simultaneous quantification of multiple analytes in a single sample. The core function of this equipment is to detect and measure the concentration of various proteins, cytokines, and other biomolecules using bead-based multiplex assays.
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Lab products found in correlation
2 protocols using bio plex 200 luminex array reader
1
Multiplexed Cytokine Profiling
2
Multiplex Immunoassay for Cytokines and Receptors
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We transported serum and lavage samples to the lab within 2 h of collection. We centrifuged the fluid sample and then subaliquoted the supernatant and serum into microtubes.
The assays used in this study were two multiplexed immunometric assay panels (Luminex platform) for cytokines (IL-1β, IL-6, IL-8, IL-10, TNF-α and IFN-γ) and for soluble receptors (sIL-2R, sIL-6R, sTNFR2 and sCD14) (R&D Systems). The Luminex xMAP system uses spectrally addressed bead sets, each of which is conjugated with a different capture monoclonal antibody specific for a given target molecule. The antibody-conjugated beads react with the sample and a secondary detection antibody to form a capture sandwich immunoassay. After completion of the assay, the Bio-Plex 200 Luminex array reader (BioRad) quantifies the amount of each analyte in the assay solution. The intraassay variations were b12%. The interassay coefficients of variance were the following: 19.95 (IL-6), 9.32 (IL-8), 27.28 (IL-10), 17.81 (TNF-α), 25.69 (IFN-γ), 25.26 (IL-1β), 6.86 (CD14), 4.88 (TNF), 3.73 (IL-6) and 7.96 (IL-2). Reporting of the cytokines was in pg/ml.
The assays used in this study were two multiplexed immunometric assay panels (Luminex platform) for cytokines (IL-1β, IL-6, IL-8, IL-10, TNF-α and IFN-γ) and for soluble receptors (sIL-2R, sIL-6R, sTNFR2 and sCD14) (R&D Systems). The Luminex xMAP system uses spectrally addressed bead sets, each of which is conjugated with a different capture monoclonal antibody specific for a given target molecule. The antibody-conjugated beads react with the sample and a secondary detection antibody to form a capture sandwich immunoassay. After completion of the assay, the Bio-Plex 200 Luminex array reader (BioRad) quantifies the amount of each analyte in the assay solution. The intraassay variations were b12%. The interassay coefficients of variance were the following: 19.95 (IL-6), 9.32 (IL-8), 27.28 (IL-10), 17.81 (TNF-α), 25.69 (IFN-γ), 25.26 (IL-1β), 6.86 (CD14), 4.88 (TNF), 3.73 (IL-6) and 7.96 (IL-2). Reporting of the cytokines was in pg/ml.
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