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Pre hsa mir 24

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Pre-hsa-miR-24 is a synthetic precursor molecule that corresponds to the human microRNA-24 (miR-24) sequence. MicroRNAs are small, non-coding RNA molecules that play important regulatory roles in gene expression. The core function of Pre-hsa-miR-24 is to serve as a tool for studying the biological functions and molecular mechanisms of miR-24 in various experimental systems.

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Lab products found in correlation

Scrambled control, by Thermo Fisher Scientific (2 mentions) Anti hsa mir 24, by Thermo Fisher Scientific (2 mentions) Negative sirna control, by Thermo Fisher Scientific (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)

2 protocols using pre hsa mir 24

1

Transfection of Cells with miR-24 Modulators

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Transfection of all different cell types was performed using Lipofectamine RNAiMAX (Invitrogen) reagent, mixed with anti-hsa-miR-24, pre-hsa-miR-24 or scrambled controls (Ambion). For each transfection, 120 pmol of anti- or pre-miR (final concentration at transfection was 50 nM) was diluted in 250 μl Opti-MEM (Gibco). Two hours after transfection, cells were treated with IL-6 as described above and harvested 24 h later. RNA was extracted using TRIzol (Invitrogen). Successful transfection (>80% of all cells) was confirmed by visual fluorescent microscopic analysis and FACS, sorting for Cy3-labelled scr-miR control. Some experiments utilized simultaneous transfection with 150 pmol siRNA (final concentration 50 nM) directed against CHI3L1/Chi3l1, with negative siRNA controls (Ambion).
Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.
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2

Transfection and cytokine treatment

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Transfection of all different cell types was performed using Lipofectamine RNAiMAX (Invitrogen) reagent, mixed with anti-hsa-miR-24, pre-hsa-miR-24, or scrambled controls (Ambion). For each transfection, 120pmol of anti- or pre-miR (final concentration at transfection was 50 nM) was diluted in 250µl Opti-MEM (Gibco). Two hours after transfection, cells were treated with IL-6 as described above and harvested 24h later. RNA was extracted using TRIzol (Invitrogen). Successful transfection (>80% of all cells) was confirmed by visual fluorescent microscopic analysis and fluorescence-activated cell sorting (FACS), sorting for Cy3-labeled scr-miR control. Some experiments utilized simultaneous transfection with 150pmol siRNA (final concentration 50nM) directed against CHI3L1/Chi3l1, with negative siRNA controls (Ambion).
Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.
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