6,6′-Bis-bromomethyl-[3,3′]bipyridine (Bpy) was prepared using the procedure described previously (19 ). Rabbit anti-ubiquitin antibody was purchased from Thermo Scientific and rabbit anti-His6 antibody was purchased from Rockland Immunochemicals. Mouse anti-CXCR4 antibody was purchased from R&D Systems. LC-MS was performed using a Finnigan LCQ Advantage IonTrap mass spectrometry coupled with a Surveyor HPLC system. Protein liquid chromatography was run on a Phenomenex Jupiter C4 column (5 μm, 300 Å, 2.00 × 50 mm) with a flow rate of 250 μL/min and a linear gradient of 5–95% acetonitrile/H2O containing 0.1% formic acid over 30 min.
Rabbit anti ubiquitin antibody
Manufactured by Thermo Fisher Scientific
The Rabbit anti-ubiquitin antibody is a primary antibody designed to detect and bind to ubiquitin, a small regulatory protein found in eukaryotic cells. This antibody can be used for the identification and analysis of ubiquitin-tagged proteins in various applications, such as western blotting and immunoprecipitation.
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2 protocols using rabbit anti ubiquitin antibody
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Synthesis of Bpy and Protein Analysis
2
Synthesis and Analysis of Bpy Compound
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6,6′-Bis-bromomethyl-[3,3′]bipyridine
(Bpy) was prepared using the procedure described previously.19 Rabbit antiubiquitin antibody was purchased
from Thermo Scientific, and rabbit anti-His6 antibody was
purchased from Rockland Immunochemicals. Mouse anti-CXCR4 antibody
was purchased from R&D Systems. LC-MS was performed using a Finnigan
LCQ Advantage IonTrap mass spectrometry coupled with a Surveyor HPLC
system. Protein liquid chromatography was run on a Phenomenex Jupiter
C4 column (5 μm, 300 Å, 2.00 × 50 mm2)
with a flow rate of 250 μL/min and a linear gradient of 5–95%
acetonitrile/H2O containing 0.1% formic acid over 30 min.
(Bpy) was prepared using the procedure described previously.19 Rabbit antiubiquitin antibody was purchased
from Thermo Scientific, and rabbit anti-His6 antibody was
purchased from Rockland Immunochemicals. Mouse anti-CXCR4 antibody
was purchased from R&D Systems. LC-MS was performed using a Finnigan
LCQ Advantage IonTrap mass spectrometry coupled with a Surveyor HPLC
system. Protein liquid chromatography was run on a Phenomenex Jupiter
C4 column (5 μm, 300 Å, 2.00 × 50 mm2)
with a flow rate of 250 μL/min and a linear gradient of 5–95%
acetonitrile/H2O containing 0.1% formic acid over 30 min.
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