Ldh cell cytotoxicity kit

Manufactured by Roche

The LDH Cell Cytotoxicity kit is a laboratory product designed to quantitatively measure the release of lactate dehydrogenase (LDH) from damaged cells. It provides a simple and reliable method for assessing cell viability or cytotoxicity in various cell-based assays.

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LDH cytotoxicity kit LDH kit

2 protocols using ldh cell cytotoxicity kit

Cytotoxicity Assay for C. burnetii

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The inhibitor concentrations tested were the same as those used in the C. burnetii intracellular growth assays in THP-1 cells (50 μM) and the control was 0.1% DMSO. LDH release was measured after incubating cells with inhibitors for 4 h (representing the day 0 infection time point), 28 h (day 1), 76 h (day 3) and 124 h (day 5) using the Roche LDH Cell Cytotoxicity kit (#11644793001), following the manufacturer’s instructions. At each indicated time point, a positive control (Triton X-100) and negative control (media only) were included in in triplicate. All results are presented as the mean of three independent experiments containing two technical repeats. Cytotoxicity was calculated as the percentage of lactate dehydrogenase (LDH) measured in Triton X-100 for that assay and are represented relative to the control (0.1% DMSO).

Debowski A.W., Bzdyl N.M., Thomas D.R., Scott N.E., Jenkins C.H., Iwasaki J., Kibble E.A., Khoo C.A., Scheuplein N.J., Seibel P.M., Lohr T., Metters G., Bond C.S., Norville I.H., Stubbs K.A., Harmer N.J., Holzgrabe U., Newton H.J, & Sarkar-Tyson M. (2023). Macrophage infectivity potentiator protein, a peptidyl prolyl cis-trans isomerase, essential for Coxiella burnetii growth and pathogenesis. PLOS Pathogens, 19(7), e1011491.

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Cytotoxicity Assay of Cell Lines

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Inhibitor-induced cytotoxicity of J774A.1 macrophage cells, Detroit 562 epithelial cells and RAW264.7 macrophage cells was measured after a 24 h incubation using the Roche LDH Cell Cytotoxicity kit (#11644793001), following the manufacturer’s instructions. All results are presented as the means of five independent experiments for J774A.1 cells and three experiments for Detroit 562 and RAW264.7 cells containing two technical repeats. A positive control (Triton X-100) and negative control (media only) were included in every assay in triplicate. Results are expressed as the percentage of lactate dehydrogenase (LDH) measured in Triton X-100 for that assay.

Iwasaki J., Lorimer D.D., Vivoli-Vega M., Kibble E.A., Peacock C.S., Abendroth J., Mayclin S.J., Dranow D.M., Pierce P.G., Fox D I.I.I., Lewis M., Bzdyl N.M., Kristensen S.S., Inglis T.J., Kahler C.M., Bond C.S., Hasenkopf A., Seufert F., Schmitz J., Marshall L.E., Scott A.E., Norville I.H., Myler P.J., Holzgrabe U., Harmer N.J, & Sarkar-Tyson M. (2022). Broad-spectrum in vitro activity of macrophage infectivity potentiator inhibitors against Gram-negative bacteria and Leishmania major. Journal of Antimicrobial Chemotherapy, 77(6), 1625-1634.

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