Inveon combined micro pet ct scanner

¹⁸F-FDG was synthesized by the PET/CT center in our institution. Following an overnight fast, mice were injected with ~0.1 mCi ¹⁸F-FDG via the tail vein. After 1 h, mice were anesthetized with 3% isoflurane inhalation and placed in the prone position in the center of a Siemens Inveon combined micro-PET/CT scanner (Siemens Preclinical Solution USA, Inc., Knoxville, TN, USA). Micro-CT scans were performed with a 80 kV X-ray tube voltage, 500 µA current, 150 ms ec exposure time and 120 rotation steps. Micro-PET static acquisition was subsequently performed for 10 min and the data were processed using the ordered set expectation maximization algorithm for three dimensional PET reconstruction. Micro-PET/CT images were analyzed with an Inveon Research Workplace 4.1 (Siemens, Erlangen, Germany). The maximum standardized uptake value (SUVmax; g/ml) of the xenograft was measured on day 0, 14 and 28 and referred to as SUVmax₀, SUVmax₁₄, SUVmax₂₈, respectively. The ¹⁸F-FDG uptake attenuation rate (FUAR) of the xenograft on day 14 and 28 was defined as FUAR₁₄ and FUAR₂₈, respectively, using the following equation: FUAR=(mean SUVmax_c value-mean SUVmax_t value)/mean SUVmax_c value ×100%, where SUVmax_c was the control group SUVmax, and the SUVmax_t was the treatment group SUVmax.

¹⁸F-FDG was synthesized by the PET/CT Center in our institution. Following an overnight fast, mice were injected with ~0.1 mCi ¹⁸F-FDG via the tail vein. After 1 h, mice were anesthetized with 3% isoflurane inhalation and placed in the prone position in the center of a Siemens Inveon combined micro-PET/CT scanner (Siemens Preclinical Solution USA, Inc., Knoxville, TN, USA). Micro-CT scans were performed with an 80 kV X-ray tube voltage, 500 μA current, 150 ms exposure time, and 120 rotation steps. Micro-PET static acquisition was subsequently performed for 10 min and the data were processed using the ordered set expectation maximization algorithm for three-dimensional PET reconstruction. Micro-PET/CT images were analyzed with an Inveon Research Workplace 4.1 (Siemens, Erlangen, Germany). The maximum standardized uptake value (SUVmax; g/ml) of the xenograft was measured before and 28 days after treatment, and the ratio of SUVmax (R_SUVmax) was calculated according to the formula of R_SUVmax=left SUVmax/right SUVmax.