Trunk blood from each dietary group (CTRL, LR, and HR, n = 6 per group) was collected into K3EDTA tubes, centrifuged at 3,000 × g for 15 min at 4 ± 2°C, and then plasma was stored at−20 °C till analysis. To avoid breakdown of active ghrelin which is extremely unstable in plasma, trunk blood was collected into K3EDTA tubes previously prepared by adding enough of the protease inhibitor 4-benzenesulfonyl fluoride HCl (TE Pro Service, Italy). Plasma levels were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's protocols [EZRL-83K/Rat Leptin ELISA, EMD Millipore, St. Charles, MI, USA; EZRGRA-90K Rat/Mouse Ghrelin (active) ELISA, EMD Millipore, St. Charles, MI, USA; Corticosterone Elisa Kit ADI-900-097, Enzo Life Sciences, Lausen, Switzerland].
Corticosterone elisa kit adi 900 097
Manufactured by Enzo Life Sciences
Sourced in United States
The Corticosterone Elisa Kit ADI-900-097 is a competitive immunoassay for the quantitative determination of corticosterone levels in biological samples. It utilizes a microplate pre-coated with a corticosterone-specific antibody and a horseradish peroxidase (HRP)-labeled corticosterone conjugate for detection.
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Lab products found in correlation
2 protocols using corticosterone elisa kit adi 900 097
1
Plasma Hormone Measurement Protocol
2
Comparative Analysis of Endocrine Biomarkers
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Following ELISA kits were used: Corticosterone ELISA kit ADI 900097 (Enzo Life Science); Aldosterone ELISA kit ADI 900173 (Enzo Life Science), ACTH ELISA kit 40–101–325001 (GenWay Biotech, Inc), cAMP EIA Kit Cat No 581001 (Cayman Chemical).
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