Rabbit polyclonal anti erk1 and anti erk2

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit polyclonal anti-ERK1 and anti-ERK2 is a laboratory reagent produced by Santa Cruz Biotechnology. It is designed to detect and bind to the ERK1 and ERK2 proteins in biological samples.

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Lab products found in correlation

2 protocols using rabbit polyclonal anti erk1 and anti erk2

Immunoblotting Analysis of Cellular Signaling

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All primary and secondary antibodies were diluted in PBS Tween-20 0.1% containing BSA 2%. Primary antibodies used were: rabbit polyclonal anti-HADHA (Proteintech, Cat# 10758-1-ap) 1:500; rabbit polyclonal anti-SQSTM1/p62 (Novus, Cat# NBP-48320, Centennial, CO, USA) 1:4000; mouse monoclonal anti-phosphoERK1/2 (Santa Cruz, Cat# 7383, Dallas, TX, USA) 1:500; rabbit polyclonal anti-ERK1 and anti-ERK2 (Santa Cruz, Cat# sc-93 and Cat# sc-154) 1:500; rabbit polyclonal anti-p21 (Proteintech, Cat# 10355-1-AP) 1:800; rabbit polyclonal anti-LC3 I/II (Novus, Cat# NB100-2220) 1:1000; rabbit monoclonal anti-phospho4EBP1 (Cell Signaling Technology, Cat# 2855T, Danvers, MA, USA) 1:500; mouse monoclonal anti-4EBP1 (Proteintech, Cat# 60246-1-1g) 1:500; mouse monoclonal anti-β Actina (Sigma Aldrich, Cat# A5441) 1:10,000.
Goat polyclonal anti-mouse 1:15,000 (Santa Cruz, Cat# sc-2005) and anti-rabbit 1:15,000 (Santa Cruz, Cat# sc-2004) were used as secondary antibodies.

Santarelli R., Pompili C., Gilardini Montani M.S., Evangelista L., Gonnella R, & Cirone M. (2022). 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells. Pharmaceutics, 14(3), 663.

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Protein Expression Evaluation Protocol

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To evaluate the expression of proteins, we used the following antibodies: mouse monoclonal anti-PARP1 (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc.), mouse monoclonal anti-p21 (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-MVK (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-pERK (1:200) (Santa Cruz Biotechnology Inc.), rabbit polyclonal anti-ERK1 and anti-ERK2 (1:200) (Santa Cruz Biotechnology Inc.), rabbit polyclonal anti-BIP (1:1000) (Cell Signaling) and mouse monoclonal anti-CHOP (1:100) (Santa Cruz Biotechnology Inc.). Mouse monoclonal anti-β-actin (1:10,000) (Novus Biological) was used as loading control. The goat anti-mouse IgG-Horseradish Peroxidase (Santa Cruz Biotechnology Inc.) and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology Inc.) were used as secondary antibodies. All the primary and secondary antibodies were diluted in PBS—0.1% Tween20 solution containing 3% of BSA (SERVA).

Romeo M.A., Gilardini Montani M.S., Benedetti R., Garufi A., D’Orazi G, & Cirone M. (2020). PBA Preferentially Impairs Cell Survival of Glioblastomas Carrying mutp53 by Reducing Its Expression Level, Stabilizing wtp53, Downregulating the Mevalonate Kinase and Dysregulating UPR. Biomolecules, 10(4), 586.

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